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Pathological investigations of Vibrio vulnificus infection in Genetically Improved Farmed Tilapia (Oreochromis niloticus L.) cultured at a floating cage farm of India

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Title Pathological investigations of Vibrio vulnificus infection in Genetically
Improved Farmed Tilapia (Oreochromis niloticus L.) cultured at a floating
cage farm of India
Not Available
 
Creator Suja,G
Sharma,S R Krupesha
Sayooj,P
Nair,V Anusree
Reshma,K J
Amala,P V
Joseph,Shoji
Sanil,N K
Sumithra,T G
 
Subject Vibrio vulnificus
vvhA gene
pilF polymorphism
GIFT
Cage farm
 
Description Not Available
An outbreak of skin ulcers with morbidity and mortality rate of 80% and 20% respectively, among Genetically Improved Farmed Tilapia (GIFT) sub-adults reared in a brackish water floating cage farm of Kochi, Kerala, India was reported. Bacterial isolation attempts from various internal organs (blood, kidney, liver, spleen and brain) of different diseased fish indicated a common bacterial etiology. Organisms were identified as Vibrio vulnificus based on conventional microbiological methods followed by molecular confirmation. Additionally, V. vulnificus marker specific for fish virulence was present in the isolates. Fish pathogenicity was confirmed by challenge tests. LD50 was calculated as 2.1 × 105.13 CFU/fish and 2.1 × 106.12 CFU/mL in injection and immersion route respectively. pilF polymorphism-based PCR and vvhA gene sequence indicated potential danger for humans. The strain was positive for siderophore, hemolysin, capsule, polar and lateral flagella. The strain could multiply rapidly in healthy fish serum. All these results indicated that the isolate could act as a primary pathogen for tilapia fish. Interestingly, serum survival ratio of the pathogen was significantly larger at higher temperatures (p ≤ .05); which may explain the increase in infective capacity of V. vulnificus at higher temperature. Multiple antibiotic resistance index of the strain was ≤0.2. In conclusion, the paper provides detailed descriptions of clinical signs, microscopic and macroscopic lesions of a natural disease outbreak among tilapia raised in brackish water cage farms of India. The concurrent attempts to identify and characterize the etiology fetches the basis for future development of effective control and preventive measures against V. vulnificus, the most infectious and lethal of all zoonotic vibrios.
Not Available
 
Date 2021-09-18T04:35:05Z
2021-09-18T04:35:05Z
2019
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64313
 
Language English
 
Relation Not Available
 
Publisher Elsevier