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Hemolymph of healthy Doclea rissoni Leach, 1815: A pool for Vibrionaceae?

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Relation http://eprints.cmfri.org.in/16533/
http://mbai.org.in/php/journaldload.php?id=2641&bkid=129
10.6024/jmbai.2022.64.2.2319-12
 
Title Hemolymph of healthy Doclea rissoni Leach, 1815: A pool for
Vibrionaceae?
 
Creator Reshma, K J
Sumithra, T G
Nair, V Anusree
Christo, J P
Drisya, D
Sanil, N K
 
Subject Invertebrates
Microbiology
 
Description As diseases have a great impact on the population dynamics,
evolution and immune biology of affected organisms, it is
important to understand the modes and mechanisms of interaction
among the lower animals and their microbial symbionts in nature
function together for survival. To achieve this, it is important to
understand the community structure of the native flora of these
animals. Spider crabs are well known for their tolerance to various
pollutants. Nevertheless, the microbiology of their circulatory
systems and their role in disease transmission are unknown.
Therefore, the bacteria associated with the hemolymph of a spider
crab, Doclea rissoni in its natural ecosystem was characterized.
Nine healthy crabs were collected from the coastal areas of Kochi,
and their hemolymphs were collected. Enumeration of viable
bacteria using various media revealed a similar count in all the
media (7-10×102 CFU/ml). Characterization of representative
isolates by conventional microbiological methods and 16S rRNA
gene sequencing was followed. Despite using three different
media, all isolates belonged to a single family Vibrionaceae. There
were two genera as Vibrio (86.67%) and Photobacterium (13.33%).
Among Vibrio, five species belonging to three different clades were
isolated, of which two could not be assigned to any known
species. These were classified as novel species (belonging to
Harveyi clade and, Brasiliensis clade) and their description is
underway. The order of relative abundance
was V. alginolyticus>Novel Vibrio-II>P. damselae>V. furnissii /
V. parahaemolyticus / Novel Vibrio-I. Of these, V. alginolyticus, P.
damselae and V. furnissii are implicated in occasional disease
outbreaks in humans and animals, posing an increased risk to
human economic activity, notably aquaculture practices. Therefore,
further investigations are required to find the basis for the
mutualistic relationship of vibrios in different crabs.
 
Publisher Marine Biological Association of India
 
Date 2022
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/16533/1/Journal%20of%20Marine%20Biological%20Association%20of%20India_2022_T.%20G.%20Sumithra.pdf
Reshma, K J and Sumithra, T G and Nair, V Anusree and Christo, J P and Drisya, D and Sanil, N K (2022) Hemolymph of healthy Doclea rissoni Leach, 1815: A pool for Vibrionaceae? Journal of the Marine Biological Association of India, 64 (2). pp. 89-93. ISSN 2321-7898