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Sequence analysis and identification of new variations in the coding sequence of melatonin receptor gene (MTNR1A) of Indian Chokla sheep breed

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Title Sequence analysis and identification of new variations in the coding sequence of melatonin receptor gene (MTNR1A) of Indian Chokla sheep breed
Not Available
 
Creator Vijay Kumar Saxena, Bipul Kumar Jha, Amar Singh Meena and S.M.K. Naqvi
 
Subject Genotyping, Polymorphism, Seasonality, Anestrus
 
Description Full length paper
Melatonin receptor 1A gene is the prime receptor mediating the effect
of melatonin at the neuroendocrine level for control of seasonal
reproduction in sheep. The aims of this study were to examine the
polymorphism pattern of coding sequence of MTNR1A gene in Chokla
sheep, a breed of Indian arid tract and to identify new variations in
relation to its aseasonal status. Genomic DNAs of 101 Chokla sheep
were collected and an 824 bp coding sequence of Exon II was
amplified. RFLP was performed with enzyme RsaI and MnlI to assess
the presence of polymorphism at position C606T and G612A,
respectively. Genotyping revealed significantly higher frequency of
M and R alleles than m and r alleles. RR and MM were found to be
dominantly present in the group of studied population. Cloning and
sequencing of Exon II followed by mutation/polymorphism analysis
revealed ten mutations of which three were non-synonymous
mutations (G706A, C893A, G931C). G706A leads to substitution of
valine by isoleucine Val125I (U14109) in the fifth transmembrane
domain. C893A leads to substitution of alanine by aspartic acid in the
third extracellular loop. G931C mutation brings about substitution of
amino acid alanine by proline in the seventh transmembrane helix,
can affect the conformational stability of the molecule. Polyphen-2
analysis revealed that the polymorphism at position 931 is potentially
damaging while the mutations at positions 706 and 893 were benign.It is concluded that G931C mutation of MTNR 1A gene, may explain,
in part, the importance of melatonin structure integrity in influencing
seasonality in sheep.
Department of Biotechnology, Government of India (Grant/Project No. BT/PR14462/AAQ/01/442/2010)
 
Date 2020-09-15T10:41:53Z
2020-09-15T10:41:53Z
2014-06-28
 
Type Article
 
Identifier http://krishi.icar.gov.in/jspui/handle/123456789/41303
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER Meta Gene, 2 (2014); 450-458