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Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-vitro fertility in bubaline spermatozoa.

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Title Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-vitro fertility in bubaline spermatozoa.
Not Available
 
Creator Rajoriya J.S.
Prasad J. K.
Ramteke S. S.
Perumal P.
De A.K.
Ghosh S. K.
Bag S.
Raje A.,
MAHAK SINGH
Kumar A.
Kumaresan A.
 
Subject EXOGENOUS CHOLESTEROL
CRYOCAPACITATION
MEMBRANE FLUIDITY
SPERMATOZOA
Buffalo bull
 
Description Not Available
A study was conducted to determine the optimum dosage of the exogenous cholesterol-
loaded cyclodextrins (CLC) to get maximum cryoprotection for bubaline spermatozoa.
In the present study, 120 × 106 spermatozoa were incubated in 2, 3 and
4 mg of CLC as grouped as Gr II, III and IV, respectively, and sperm progressive motility,
intracellular Ca2+, capacitation status by protein tyrosine phosphorylation (PTP)
assay and zona binding per cent (ZBP) and cleavage rate (CR) of the cryopreserved
buffalo spermatozoa by in vitro fertility assay were assessed in comparison with an
untreated control group (Gr I). Results revealed that there was a significant (p < .05)
linear decrease in percentage of sperm population with higher intracellular Ca2+ and
percentage of sperm population with medium or high capacitated by PTP in CLC
treated from 2 to 3 mg and then increased to 4 mg/120 × 106 spermatozoa whereas
sperm progressive motility, percentage of sperm population with low capacitated,
ZBP and CR were increased significantly (p < .05) in sperm population treated from
2 to 3 mg CLC and then decreased to 4 mg/120 × 106 spermatozoa. The study has
clearly indicated that CLC at 3 mg/120 × 106 spermatozoa has maximum beneficial
effects in protection of sperm progressive motility, membrane fluidity (low intracellular
Ca2+); prevention of cryocapacitation (low capacitation pattern in immunolocalization)
and enhancement of in vitro ZBP and CR. Post-thaw motility of the CLC-treated
sperm has shown positively significant (p < .05) correlation with sperm population
with low intracellular Ca2+, low capacitated sperm population, ZBP and CR, whereas
it was negatively (p < .05) correlated with sperm population with high intracellular
Ca2+, medium or high capacitated sperm. The present study has revealed for the
first time that incubation of spermatozoa with CLC of higher dose (>3 mg/120 × 106
spermatozoa) had adverse effects on sperm cryopreservation, although incubation of
sperm with 3 mg/120 million prior to processing had minimised the freezing–thawing-
associated damages in bubaline species.
Not Available
 
Date 2021-09-29T10:13:53Z
2021-09-29T10:13:53Z
2020-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64811
 
Language English
 
Relation Not Available;
 
Publisher Not Available