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Conversion of normal inbred lines into quality protein maize through marker assisted selection

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Title Conversion of normal inbred lines into quality protein maize through marker assisted selection
Not Available
 
Creator Srivastava S
Gangoliya SS
Yathish KR
Kumar A
Preeti
Goshal T
Yadav HK
Kapoor U
Kumar S
Kumar R
Singh SB
Sekhar JC
Das AK
Neelam S
Yadav OP
Mahajan V
Karjagi CG
Rakshit S
 
Subject QPM
 
Description Not Available
Protein malnutrition is one of the challenges to ensure nutritional security of the
global poor masses especially in rural population of the developing world. Cereals
are deficient in some of essential amino acids like lysine and tryptophan. The
deficiency leads to one of the protein deficiency disorder like kwashiorkor. Therefore
the challenge is to deliver nutritious, safe and affordable food to reduce the impact of
nutritional deficiency. Quality protein maize (QPM), contain double the amount of
lysine and tryptophan as compared to normal maize is one of the intervention could
play vital role to reduce amino acid deficiency. In this context, several efforts have
been made to enhance the lysine and tryptophan levels through marker assisted
introgression of o2 gene into elite inbred lies of maize. In the present study, opaque2
gene along with phenotypic selection for kernel modification was incorporated using
marker assisted backcross breeding (MABB) program into normal inbred line i.e. LM19. This is the parental line of popular single cross hybrids PMH6 in India. HKI
163 was the donor for opaque2 allele. opaque2 gene􀀐based simple sequence repeat
(SSR) markers were successfully used for introgression of opaque2 allele. BC2F2
population derived from marker identified BC2F1 individuals were subjected to
foreground selection. 86 BC2F2 plants derived from LM19 × HKI 163 cross were
screened for o2 gene linked molecular marker. The result showed that 50 plants were
heterozygous, 18 plants were homozygous while 15 plants were negative for o2 allele.
The o2 carrying homozygous plants were subjected to background selection using
sequence tagged microsatellites (STMS) to accelerate the recovery of recurrent parent
genome (RPG). The background selection has revealed that the RPG varied from 79􀀐
87 % in BC2F2 generation derived from LM19 × HKI 163 cross. The newly developed
near isogenic lines (NILs) from the hard endosperm kernels were evaluated for
desirable agronomic and biochemical traits in replicated trials and the best lines were
chosen to represent the QPM version of LM19. The agronomic performance of the
selected BC2F2 population for LM19 × HKI 163 inbreds showed comparable
performance with LM17 in days of anthesis, days of silking and yield and yield
component traits.
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Date 2021-01-25T09:26:47Z
2021-01-25T09:26:47Z
2020-02-09
 
Type Proceedings
 
Identifier In proceedings of National Seminar on “Maize for Crop Diversification under Changing Climatic Scenario” is being organized on 9 & 10 February 2020 by Maize Technologist Association of India, ICAR-Indian Institute of Maize Research, Ludhiana in collaboration of Punjab Agricultural University, Ludhiana
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/45037
 
Language English
 
Relation Not Available;
 
Publisher Not Available