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Introgression of crtRB1 through marker assisted backcross breeding (MABB)

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Title Introgression of crtRB1 through marker assisted backcross breeding (MABB)
Not Available
 
Creator Yadav HK
Gangoliya SS
Yathish KR
Kumar A
Preeti
Goshal T
Srivastava S
Kapoor U
Kumar S
Kumar R
Singh SB
Sekhar JC
Das AK
Neelam S
Yadav OP
Mahajan V
Karjagi CG
 
Subject Provitamin A
 
Description Not Available
Maize (Zea mays L.) is grown on nearly 190 million ha in about 165 countries with
total production yielding approximately 39% of total grain production. In India maize
is third most important cereal crop after rice and wheat and accounts for around 10%
of total food grain production. Maize contributes 25 to 35 per cent of total calories of
the diets in several African, Asian and Latin American countries. Thus it is a valuable
source of food and feed worldwide. Further enhancement of nutritional value through
micronutrients like provitamin A in maize through biofortification could play an vital
role in reducing the micronutrient malnutrition, the major challenge to ensure
nutritional security in developing countries. Biofortification is the process by which
the nutrient density of food crops is increased through conventional plant
breeding and/or improved agronomic practices and/or modern biotechnology without
sacrificing any characteristic that is preferred by consumers or most importantly to
farmers. In the present study, gene encoding β-carotene hydroxylase 1 (crtRB1)
alleles associated with higher β-carotene concentrations was introgressed into elite
inbred line LM 17 and 19 using marker-assisted backcross breeding (MABB). The
donor for crtRB1 gene was CIMMYT􀀐5 inbred line. For foreground selection crtRB1
gene-based simple sequence repeat (SSR) markers were successfully used in
foreground selection. Presently introgressed lines are in BC2F2 generation. In LM17 ×
CIMMYT􀀐5 cross, out of 90 plants, 31 plants were homozygous, 42 plants were
heterozygous while 17 plants were negative and in LM19 × CIMMYT􀀐5 cross out of
167 plants, 54 plants were homozygous, 87 plants were heterozygous while 26 plants
were negative. The plants carrying gene of interest, were screened with SSR markers
and recovery of recurrent parent genome (RPG) in background selection ranged
between 82.40 - 93.70% for the LM17 × CIMMYT􀀐5 and 84.70􀀐91.30% for LM19 ×
CIMMYT􀀐5. The newly developed provitamin A near siogenic lines (NILs)
developed in both the crosses are comparable with their recurrent parents for
agronomic performance for different traits like days of anthesis, ear diameter, ear
height, ear length, kernels per row and kernels rows, however the biochemical
analysis is being carried out.
Not Available
 
Date 2021-01-25T09:16:35Z
2021-01-25T09:16:35Z
2020-02-09
 
Type Proceedings
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/45036
 
Language English
 
Relation Not Available;
 
Publisher Not Available