ICAR Research Data Repository for Knowledge Management
CRISPR-Cas Methods
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
CRISPR-Cas Methods
Not Available |
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| Creator |
Islam, M. Tofazzal, Bhowmik, Pankaj, Molla, Kutubuddin A. (Eds.)
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| Subject |
Genome Editing
CRISPR-Cas Precise engineering |
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| Description |
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism. Not Available |
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| Date |
2021-01-01T12:10:20Z
2021-01-01T12:10:20Z 2020-08-01 |
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| Type |
Book
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| Identifier |
Islam, M. T., Bhowmik, P. K., & Molla, K. A. CRISPR-Cas Methods.Springer, Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0616-2
https://www.springer.com/gp/book/9781071606155#aboutBook http://krishi.icar.gov.in/jspui/handle/123456789/44429 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Springer
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