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Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers

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Title Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers
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Creator Hanuman Ram
Nirmal K. Hedau
Ganesh V. Chaudhari
Mukesh Choudhary
Lakshmi Kant
 
Subject Peas, genetic diversity, SSR markers, PIC
 
Description Not Available
The present study was conducted on genetic diversity analyses among 24 pea
genotypes during 2017–2018 to assess the molecular diversity of pea genotypes
using SSR markers. Out of 62, eleven markers were found to be polymorphic
and the polymorphic information content (PIC) of the simple sequence repeat
(SSR) markers ranged from 0.19 to as high as 0.64. Molecular profiling of these
genotypes using 11 SSRs distributed throughout the genome generated 32 alleles
with a mean of 2.91 alleles per locus. The genetic dissimilarity based on simple
matching coefficient for 24 genotypes ranged from 0.00 to 0.91 with an average
of 0.52. Cluster analyses grouped 24 genotypes into two major clusters with one
outlier and supported by principal coordinate analysis (PCoA) in which genotypes
were distributed across four quadrangles. Analysis of molecular variance (AMOVA)
showed significant estimated value at degree of 1000 permutations. Percentage
of variability was higher among individual (67%) than among populations (11%).
Percentage of variability within individual was also higher (22%) than among
populations (11%). Pop1 (I=0.707, He=0.446, and uHe=0.466) shows higher
diversity than pop2 (I=0.630, He=0.381 and uHe=0.398). The percentage of
polymorphic loci per population (PPL) ranged from 81.82% (pop2) to 90.91%
(pop1) with an average of 86.36%. The present study demonstrates the utility
of microsatellite markers for estimating molecular diversity as well as genotype
identification in pea. This study also suggests a potential use of these markers in
further association studies.
ICAR
 
Date 2022-04-01T04:27:28Z
2022-04-01T04:27:28Z
2021-08-30
 
Type Book
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/71070
 
Language English
 
Relation Not Available;
 
Publisher Not Available