Record Details

Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice
Not Available
 
Creator Virender Jeet Singh Thakur, Revathi Ponnuswamy, Arun Kumar Singh1, V. Gouri Shankar and D. Srinivasa Chary
 
Subject Hybrid rice, Rf genes, WA-CMS system, BSA, Fertility restoration
 
Description Not Available
The process of screening for fertility restoration trait
involves test crossing with a set of cytoplasmic male sterile
(CMS) lines and evaluation of F1 hybrids for pollen and
spikelet fertility. In the present study, F2 mapping population
derived from a cross, APMS 6A × RP 5933-123 was utilized
to map Rf genes. The F2 population was also genetically
analysed for pollen and spikelet fertility percentage. Chisquare
(χ2) analysis to showed that the fertility restoration
trait followed expected digenic ratio. By bulk segregant
analysis (BSA) likely Rf genes containing regions were
located on chromosome 10. The SSR markers viz., RM304,
RM258 located on chromosome 10 and RM23958 located
on chromosome 9 showed clear polymorphism between
two groups of fertile and sterile bulks. Based on BSA linkage
analysis and F2 population, pollen and spikelet fertility
analysis along with molecular screening results of Rf linked
markers, it is concluded that Rf4 gene located on
chromosome 10 is playing major role and contributing to
90% of fertility restoration trait of newly derived restorer
line RP5933 along with minor effect genes from chromosome
9. The findings may be useful for rice hybrid breeding.
Not Available
 
Date 2022-04-05T17:47:41Z
2022-04-05T17:47:41Z
2021-01-01
 
Type Research Paper
 
Identifier 92. Thakur, V.J.S., Ponnuswamy, R., Singh, A.K., Shankar, V.G. and Chary, S.D., 2021. Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice. Indian Journal of Genetics and Plant Breeding, 81(01), pp.43-49.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/71218
 
Language English
 
Relation Not Available;
 
Publisher Not Available