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High throughput residue analysis of paraquat and diquat involving hydrophilic interaction liquid chromatographic separation and mass spectrometric determination

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Title High throughput residue analysis of paraquat and diquat involving hydrophilic interaction liquid chromatographic separation and mass spectrometric determination
Not Available
 
Creator Oulkar D., Shinde R., Khan Z., Banerjee Kaushik
 
Subject Paraquat
Diquat
Hydrophilic interaction liquid chromatography
LC-MS/MS
Residue analysis in grape, apple, pomegranate
 
Description Not Available
A selective, sensitive and robust LC-MS/MS method is reported for the determination of the residues of paraquat and diquat in various fruit matrices, including grape, apple and pomegranate. The extraction with acidified water (0.1 M HCl) at 80°C (15 min) offered superior recoveries for both analytes with a significantly lower matrix effects as compared to the extraction with acidified methanol by the methods reported in the existing literature. The optimised HPLC conditions on hydrophilic interaction liquid chromatography (HILIC) columns, when coupled with electrospray ionisation-tandem mass spectrometry, offered their limit of quantification at 0.01 mg kg−1. The analysis on an XBridge HILIC column required a thorough optimisation of the gradient programme to induce chromatographic separation and minimise matrix effects. This was not necessary when a CORTECS HILIC column was used, which provided selective and sensitive analysis within 5 min runtime using isocratic flow. Isotopically labelled internal standards corrected the recoveries of both analytes within 70–120% (RSD < 20%). For the first time, the applications of high resolution accurate mass analysis in the ‘time of flight – multiple reaction monitoring’ mode have been demonstrated as a complementary means of targeted screening of these compounds at 0.01 mg kg−1 level. The method has a strong potential for applications in both official control and by those involved in food production for checking compliance with the EU MRLs.
Not Available
 
Date 2020-07-31T06:24:58Z
2020-07-31T06:24:58Z
2019-01-03
 
Type Journal
 
Identifier Oulkar D., Shinde R., Khan Z., Banerjee Kaushik (2019). High throughput residue analysis of paraquat and diquat involving hydrophilic interaction liquid chromatographic separation and mass spectrometric determination. Food Additives & Contaminants: Part A 36 (1): 120-130
https://doi.org/10.1080/19440049.2018.1547424
http://krishi.icar.gov.in/jspui/handle/123456789/38649
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis