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Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Bubalus bubalis)

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Title Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Bubalus bubalis)
Not Available
 
Creator Parul Sarwalia
Mustafa Raza
Apoorva Soni
Pratiksha Dubey
Rajeev Chandel
Rakesh Kumar
A. Kumaresan
Suneel Kumar Onteru
Ankit Pal
Kalpana Singh
Mir Asif Iquebal
Sarika Jaiswal
Dinesh Kumar
T. K. Datta
 
Subject circulatory miRNA expression
buffalo placenta
early pregnancy detection
feto-maternal cross talk
maternal recognition of pregnancy
 
Description Not Available
Precise early pregnancy diagnosis in dairy animals is of utmost importance for an
efficient dairy production system. Not detecting a dairy animal pregnant sufficiently early
after the breeding results to extending the unproductive time of their milk production
cycle and causes substantial economic loss for a dairy producer. At present, the most
conventional and authentic pregnancy confirmation practice in cows and buffaloes
is rectal palpation of the reproductive organs at Days 35–40 after insemination,
which sometime leads to considering an animal as false pregnant. Other alternative
methods available for early pregnancy diagnosis lack either accuracy or reproducibility
or require elaborate instrumentation and laboratory setup not feasible to practice at
farmers’ doorstep. The present study was aimed at establishment of the microRNA
(miRNA) repertoire of the placentome in buffaloes, which could capture the event of
the cross talk between a growing embryo and a dam, through fetal cotyledons and
maternal caruncles, and thus could hint at the early pregnancy establishment event in
ruminants. Total RNA was isolated from buffalo placentome tissues during early stages
of pregnancy (at Day < 25 and Days 30–35), and global small RNA analysis was
performed by using Illumina single-end read chemistry and Bubalus bubalis genome.
A total of 2,199 miRNAs comprising 1,620 conserved and 579 non-conserved miRNAs
were identified. Stringent functional miRNA selection criteria could predict 20 miRNAs
worth evaluating for their abundance in the plasma of pregnant, non-pregnant, cyclic
non-bred, and non-cyclic prepubertal animals. Eight of them (viz., miR-195-5p, miR-
708-3p, miR-379-5p, miR-XX1, miR-XX2, miR-130a-3p, miR-200a-3p, and miR-27)
displayed typical abundance patterns in the plasma samples of the animals on Day
19 as well as Day 25 post-insemination, thus making them ambiguous candidates forearly pregnancy detection. Similarly, higher abundance of miR-200a-3p and miR130a-
3p in non-pregnant animals was indicative of their utility for detecting the animals as
not pregnant. Most interestingly, miR-XX1 and miR-XX2 were very characteristically
abundant only in pregnant animals. In silico target prediction analysis confirmed that
these two miRNAs are important regulators of cyclooxygenase-2 (COX-2) and cell
adhesion molecule-2 (CADM-2), both of which play a significant role in the implantation
process during feto-maternal cross talk. We interpret that circulatory miR-XX1 and miRXX2
in blood plasma could be the potential biomarkers for early pregnancy detection
in buffaloes.
Not Available
 
Date 2022-03-15T06:47:20Z
2022-03-15T06:47:20Z
2021-08-26
 
Type Research Paper
 
Identifier Sarwalia P, Raza M, Soni A, Dubey P, Chandel R, Kumar R, Kumaresan A, Onteru SK, Pal A, Singh K, Iquebal MA, Jaiswal S, Kumar D and Datta TK (2021) Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Bubalus bubalis). Front. Cell Dev. Biol. 9:673765. doi: 10.3389/fcell.2021.673765
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/70232
 
Language English
 
Relation Not Available;
 
Publisher Not Available