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Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing

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Title Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing
Not Available
 
Creator Kiran D. Rasal
Mir Asif Iquebal
Sangita Dixit
Manohar Vasam
Mustafa Raza
Lakshman Sahoo
Sarika Jaiswal
Samiran Nandi
Kanta Das Mahapatra
Avinash Rasal
Uday Kumar Udit
Prem Kumar Meher
Khuntia Murmu
UB Angadi
Anil Rai
Dinesh Kumar
Jitendra Kumar Sundaray
 
Subject Labeo rohita
transcriptome
gene ontology
KEGG
insulin signaling pathway
 
Description Not Available
Although feed cost is the greatest concern in aquaculture, the inclusion of carbohydrates in
the fish diet, and their assimilation, are still not well understood in aquaculture species. We identified
molecular events that occur due to the inclusion of high carbohydrate levels in the diets of genetically
improved ‘Jayanti rohu’ Labeo rohita. To reveal transcriptional changes in the liver of rohu, a feeding
experiment was conducted with three doses of gelatinized starch (20% (control), 40%, and 60%).
Transcriptome sequencing revealed totals of 15,232 (4464 up- and 4343 down-regulated) and 15,360
(4478 up- and 4171 down-regulated) di erentially expressed genes. Up-regulated transcripts
associated with glucose metabolisms, such as hexokinase, PHK, glycogen synthase and PGK, were found
in fish fed diets with high starch levels. Interestingly, a de novo lipogenesis mechanism was found
to be enriched in the livers of treated fish due to up-regulated transcripts such as FAS, ACC ,
and PPAR
. The insulin signaling pathways with enriched PPAR and mTOR were identified by
Kyoto Encyclopedia of Genes and Genome (KEGG) as a result of high carbohydrates. This work
revealed for the first time the atypical regulation transcripts associated with glucose metabolism and
lipogenesis in the livers of Jayanti rohu due to the inclusion of high carbohydrate levels in the diet.
This study also encourages the exploration of early nutritional programming for enhancing glucose
e ciency in carp species, for sustainable and cost-e ective aquaculture production.
Not Available
 
Date 2022-03-19T08:29:01Z
2022-03-19T08:29:01Z
2020-10-31
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/70366
 
Language English
 
Relation Not Available;
 
Publisher Not Available