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Insights Into the Host-Pathogen Interaction Pathways Through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection

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Title Insights Into the Host-Pathogen Interaction Pathways Through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection
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Creator Gyan P. Mishra
Muraleedhar S. Aski
Tejas Bosamia
Shiksha Chaurasia
Dwijesh Chandra Mishra
Jyotika Bhati
Atul Kumar
Shaily Javeria
Kuldeep Tripathi
Manju Kohli
Ranjeet Ranjan Kumar
Amit Kumar Singh
Jyoti Devi
Shiv Kumar
Harsh Kumar Dikshit
 
Subject Lens culinaris
Rhizoctonia bataticola
RNA-Seq
transcription factors
plant defense
signal pathway
 
Description Not Available
Dry root rot (Rhizoctonia bataticola) is an important disease of lentils (Lens culinaris Medik.).To gain an insight into the molecular aspects of host-pathogen interactions, the RNA-seq approach was used in lentils following inoculation with R.bataticola. The RNA-Seq has generated >450 million high-quality reads (HQRs) and nearly 96.97% were properly aligned to the reference genome. Very high similarity in FPKM (fragments per kilobase of exon per million mapped fragments) values (R > 0.9) among biological replicates showed the consistency of the RNA-Seq results. The study revealed various DEGs (differentially expressed genes) that were associated with changes in phenolic compounds, transcription factors (TFs), antioxidants, receptor kinases, hormone signals which corresponded to the cell wall modification enzymes, defense-related metabolites, and jasmonic acid (JA)/ethylene (ET) pathways. Gene ontology (GO) categorization also showed similar kinds of significantly enriched similar GO terms. Interestingly, of the total unigenes (42,606), 12,648 got assembled and showed significant hit with Rhizoctonia species. String analysis also revealed the role of various disease responsive proteins viz., LRR family proteins, LRR-RLKs, protein kinases, etc. in the host-pathogen interaction. Insilico validation analysis was performed using Genevestigator® and DEGs belonging to six major defense-response groups viz., defense-related enzymes, disease responsive genes, hormones, kinases, PR (pathogenesis related) proteins, and TFs were validated. For the first time some key miRNA targets viz. miR156, miR159, miR167, miR169, and miR482 were identified from the studied transcriptome, which may have some vital role in Rhizoctonia-based responses in lentils. The study has revealed the molecular mechanisms of the lentil/R.bataticola interactions and also provided a theoretical approach for the development of lentil genotypes resistant to R.bataticola
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Date 2022-04-16T10:22:47Z
2022-04-16T10:22:47Z
2021-12-29
 
Type Research Paper
 
Identifier Mishra, G.P.; Aski, M.S.; Bosamia, T.; Chaurasia, S.; Mishra, D.C.; Bhati, J.; Kumar, A.; Javeria, S.; Tripathi, K.; Kohli, M.; Kumar, R.R.; Singh, A.K.; Devi, J.; Kumar, S.; Dikshit, H.K. Insights into the Host-Pathogen Interaction Pathways through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection. Genes 2022, 13, 90. https://doi.org/10.3390/genes13010090
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http://krishi.icar.gov.in/jspui/handle/123456789/71533
 
Language English
 
Relation Not Available;
 
Publisher MDPI Genes