Insights Into the Host-Pathogen Interaction Pathways Through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection
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Title |
Insights Into the Host-Pathogen Interaction Pathways Through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection
Not Available |
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Creator |
Gyan P. Mishra
Muraleedhar S. Aski Tejas Bosamia Shiksha Chaurasia Dwijesh Chandra Mishra Jyotika Bhati Atul Kumar Shaily Javeria Kuldeep Tripathi Manju Kohli Ranjeet Ranjan Kumar Amit Kumar Singh Jyoti Devi Shiv Kumar Harsh Kumar Dikshit |
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Subject |
Lens culinaris
Rhizoctonia bataticola RNA-Seq transcription factors plant defense signal pathway |
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Description |
Not Available
Dry root rot (Rhizoctonia bataticola) is an important disease of lentils (Lens culinaris Medik.).To gain an insight into the molecular aspects of host-pathogen interactions, the RNA-seq approach was used in lentils following inoculation with R.bataticola. The RNA-Seq has generated >450 million high-quality reads (HQRs) and nearly 96.97% were properly aligned to the reference genome. Very high similarity in FPKM (fragments per kilobase of exon per million mapped fragments) values (R > 0.9) among biological replicates showed the consistency of the RNA-Seq results. The study revealed various DEGs (differentially expressed genes) that were associated with changes in phenolic compounds, transcription factors (TFs), antioxidants, receptor kinases, hormone signals which corresponded to the cell wall modification enzymes, defense-related metabolites, and jasmonic acid (JA)/ethylene (ET) pathways. Gene ontology (GO) categorization also showed similar kinds of significantly enriched similar GO terms. Interestingly, of the total unigenes (42,606), 12,648 got assembled and showed significant hit with Rhizoctonia species. String analysis also revealed the role of various disease responsive proteins viz., LRR family proteins, LRR-RLKs, protein kinases, etc. in the host-pathogen interaction. Insilico validation analysis was performed using Genevestigator® and DEGs belonging to six major defense-response groups viz., defense-related enzymes, disease responsive genes, hormones, kinases, PR (pathogenesis related) proteins, and TFs were validated. For the first time some key miRNA targets viz. miR156, miR159, miR167, miR169, and miR482 were identified from the studied transcriptome, which may have some vital role in Rhizoctonia-based responses in lentils. The study has revealed the molecular mechanisms of the lentil/R.bataticola interactions and also provided a theoretical approach for the development of lentil genotypes resistant to R.bataticola Not Available |
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Date |
2022-04-16T10:22:47Z
2022-04-16T10:22:47Z 2021-12-29 |
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Type |
Research Paper
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Identifier |
Mishra, G.P.; Aski, M.S.; Bosamia, T.; Chaurasia, S.; Mishra, D.C.; Bhati, J.; Kumar, A.; Javeria, S.; Tripathi, K.; Kohli, M.; Kumar, R.R.; Singh, A.K.; Devi, J.; Kumar, S.; Dikshit, H.K. Insights into the Host-Pathogen Interaction Pathways through RNA-Seq Analysis of Lens culinaris Medik. in Response to Rhizoctonia bataticola Infection. Genes 2022, 13, 90. https://doi.org/10.3390/genes13010090
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/71533 |
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Language |
English
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Relation |
Not Available;
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Publisher |
MDPI Genes
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