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Whole genome sequencing of spotted stem borer, Chilo partellus, reveals multiple genes encoding enzymes for detoxification of insecticides

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Title Whole genome sequencing of spotted stem borer, Chilo partellus, reveals multiple genes encoding enzymes for detoxification of insecticides
Not Available
 
Creator Mukesh K. Dhillon
Jagdish Jaba
Pallavi Mishra
Mir Asif Iquebal
Sarika Jaiswal
Aditya K. Tanwar
Nareshkumar Bharat
Naveen Arora
Suraj Prasad Mishra
Shyam Prasad Gogineni
Fazil Hasan
Anil Rai
Dinesh Kumar
Hari C. Sharma
 
Subject Maize
Sorghum
Spotted stem borer
Chilo partellus
Genome assembly
Insecticide detoxification
 
Description Not Available
Spotted stem borer, Chilo partellus, is the most important constraint for increasing the production and productivity of maize
and sorghum, the two major coarse cereals in Asia and Africa. The levels of resistance to this pest in the cultivated germplasm
are low to moderate, and hence, farmers have to use insecticides for effective control of this pest. However, there is no
information on the detoxification mechanisms in C. partellus, which is one of the constraints for deployment of appropriate
insecticides to control this pest. The ability to detoxify insecticides varies across insect populations, and hence, we sequenced
different populations of C. partellus to identify and understand detoxification mechanisms to devise appropriate strategies
for deployment of different insecticides for controlling this pest. Larval samples were sequenced from three different cohorts
of C. partellus using the Illumina HiSeq 2500 platform. The data were subjected to identify putative genes that are involved
in detoxification on insecticides in our cohort insect species. These studies resulted in identification of 64 cytochrome P450
genes (CYP450s), and 36 glutathione S-transferases genes (GSTs) encoding metabolic detoxification enzymes, primarily
responsible for xenobiotic metabolism in insects. A total of 183 circadian genes with > 80% homolog and 11 olfactory receptor
genes that mediate chemical cues were found in the C. partellus genome. Also, target receptors related to insecticide
action, 4 acetylcholinesterase (AChE), 14 γ-aminobutyric acid (GABA), and 15 nicotinic acetylcholine (nAChR) receptors
were detected. This is the first report of whole genome sequencing of C. partellus useful for understanding mode of action
of different insecticides, and mechanisms of detoxification and designing target-specific insecticides to develop appropriate
strategies to control C. partellus for sustainable crop production.
Not Available
 
Date 2022-04-21T10:23:40Z
2022-04-21T10:23:40Z
2022-04-15
 
Type Research Paper
 
Identifier Dhillon, M.K., Jaba, J., Mishra, P. et al. Whole genome sequencing of spotted stem borer, Chilo partellus, reveals multiple genes encoding enzymes for detoxification of insecticides. Funct Integr Genomics (2022). https://doi.org/10.1007/s10142-022-00852-w
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http://krishi.icar.gov.in/jspui/handle/123456789/71654
 
Language English
 
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Publisher Not Available