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Promoter sequence interaction and structure based multi-targeted (redox regulatory genes) molecular docking analysis of vitamin E and curcumin in T4 induced oxidative stress model using H9C2 cardiac cell line

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Title Promoter sequence interaction and structure based multi-targeted (redox regulatory genes) molecular docking analysis of vitamin E and curcumin in T4 induced oxidative stress model using H9C2 cardiac cell line
Not Available
 
Creator Pallavi Mishra
Gitanjali Tandon
Manoj Kumar
Biswaranjan Paital
Shasanka Sekhar Swain
Sunil Kumar
Luna Samanta
 
Subject Promoter ligand interaction
curcumin; vitamin E
structural binding
NRF-2/ KEAP 1 activation
 
Description Not Available
A positive association between oxidative stress and hyper-thyroid conditions is well established.
Vitamin E (VIT-E) and curcumin (CRM) are considered as potent antioxidant small molecules. Nuclear
factor erythroid 2–related factor 2(NRF-2) is known to bind with antioxidant response element and
subsequently activate expression of antioxidant enzymes. However, the activation of NRF-2 depends
on removal of its regulator Kelch-like ECH-associated protein 1(NRF-2). In the current study, an attempt
is made to demonstrate whether effects of VIT-E and CRM are due to direct interaction with the target
proteins (i.e. NRF-2, NRF-2, SOD, catalase and LDH) or by possible interaction with the flanking region
of their promoters by in silico analysis. Further, these results were corroborated by pretreatment of
H9C2 cells (1 x 106 cells per mL of media) with VIT-E (50 lM) and/or CRM (20 lM) for 24 h followed by
induction of oxidative stress via T4 (100 nm) administration and assaying the active oxygen metabol ism. Discriminant function analyses (DFA) indicated that T4 has a definite role in increasing oxidative
stress as evidenced by induction of ROS generation, increase in mitochondrial membrane potential
and elevated lipid peroxidation (LPx). Pretreatment with the two antioxidants have ameliorative effects
more so when given in combination. The decline in biological activities of the principal antioxidant
enzymes SOD and CAT with respect to T4 treatment and its restoration in antioxidant pretreated
group further validated our in silico data.
Not Available
 
Date 2022-05-20T09:27:11Z
2022-05-20T09:27:11Z
2021-08-01
 
Type Research Paper
 
Identifier Pallavi Mishra, Gitanjali Tandon, Manoj Kumar, Biswaranjan Paital, Shasanka Sekhar Swain, Sunil Kumar & Luna Samanta (2021): Promoter sequence interaction and structure based multi-targeted (redox regulatory genes) molecular docking analysis of vitamin E and curcumin in T4 induced oxidative stress model using H9C2 cardiac cell line, Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2021.1970624
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72345
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis