Record Details

In vitro propagation of almond (Prunus dulcis) cv. Merced

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title In vitro propagation of almond (Prunus dulcis) cv. Merced
 
Creator MIR, M A
BHAT, K M
RATHER, Z A
P, F A
PANDIT, A H
Hussain, G
 
Subject Almond; Growth regulators; Micro propagation; Proliferation; Rooting
 
Description Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Surface sterilization of explants with mercuric chloride 0.1% (w/v) for 10 min. was found effective in improving culture asepsis (51.66%) and explant survival (55.00%). Higher values for both these parameters were recorded with forced explants in comparison to unforced ones. Main effect of growth regulators and media was significant on explant establishment which was maximum (66.66%) on ½ MS media containing BAP + IBA (0.50+0.01 mg/l). Callusing at the base of initiating cultures was minimum (24.58%) with BAP+IBA (0.25+0.01 mg/l). Microshoots from the established cultures were subcultured on the MS media supplemented with BAP and NAA alone or in combination for axillary shoot proliferation. Maximum proliferated cultures (86.66%) with maximum shoot number/explant (15.61) and proliferation grade (4.00) was obtained with BAP+NAA ( 0.40 + 0.01 mg/l). BAP was found superior to NAA during axillary shoot proliferation. Microshoots (10–15 mm) from proliferated cultures were subcultured in root induction medium (MS medium supplemented with IBA) and incubated under darkness for 10 days at 24±1 degree Celsius and then transferred to root development medium (hormone-free MS medium) and incubated under normal culture room conditions. Highest rooting of microshoots (93.33%) with maximum root number/shoot (5.90) and root length (43.00 mm) was obtained with IBA (1.0 mg/l).
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2012-04-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/16632
 
Source The Indian Journal of Agricultural Sciences; Vol 82, No 4 (2012)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/16632/8027
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences