Record Details

Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
Not Available
 
Creator V. Dinkar, S. K. Jha, N. Mallick, M. Niranjana, P. Agarwal, J. B. Sharma and Vinod
 
Subject Wheat, Triticum spelta, Puccinia triticina, SSR, SNP, Mapping
 
Description Not Available
TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad
spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and
BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive
gene for leaf rust resistance, tentatively named as LrTs276-2, in TSD276-2. Molecular mapping of leaf
rust resistance gene LrTs276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For
Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents
TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders’ AXIOM
array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust
resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome
arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm
1DS were also identifed. Final linkage map was constructed using one SNP-based PCR and three
SSR markers. The rust reaction and chromosomal location suggest that LrTs276-2 is a new leaf rust
resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.
NAHEP
 
Date 2022-11-10T05:52:26Z
2022-11-10T05:52:26Z
2020-12-17
 
Type Research Paper
 
Identifier Dinkar, V., Jha, S.K., Mallick, N., Niranjana, M., Agarwal, P. and Sharma, J.B., 2020. Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta. Scientific reports, 10(1), pp.1-9.e
https://doi.org/10.1038/s41598-020-78679-3
http://krishi.icar.gov.in/jspui/handle/123456789/74985
 
Language English
 
Relation Not Available;
 
Publisher Nature