Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
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Title |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
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Creator |
V. Dinkar, S. K. Jha, N. Mallick, M. Niranjana, P. Agarwal, J. B. Sharma and Vinod
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Subject |
Wheat, Triticum spelta, Puccinia triticina, SSR, SNP, Mapping
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Description |
Not Available
TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders’ AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identifed. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat. NAHEP |
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Date |
2022-11-10T05:52:26Z
2022-11-10T05:52:26Z 2020-12-17 |
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Type |
Research Paper
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Identifier |
Dinkar, V., Jha, S.K., Mallick, N., Niranjana, M., Agarwal, P. and Sharma, J.B., 2020. Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta. Scientific reports, 10(1), pp.1-9.e
https://doi.org/10.1038/s41598-020-78679-3 http://krishi.icar.gov.in/jspui/handle/123456789/74985 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Nature
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