Record Details

Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber
Not Available
 
Creator Neha Kumari Mandal
Khushboo Kumari
Aditi Kundu
Ajay Arora
Prolay K. Bhowmick
Mir Asif Iquebal
Sarika Jaiswal
Tushar Kanti Behera
A.D. Munshi
Shyam S. Dey
 
Subject cucumber
parthenocarpy
slicing
pickling cucumber
cytokinin
gene expression
endogenous level
exogenous spray
 
Description Not Available
Cucumber is a model plant for studying parthenocarpy with abundant slicing- and pickling-type germplasm. This study was undertaken to understand the role of the important cytokines (CKs), auxin (AUX) and gibberellin (GA) biosynthesis and degradation genes for the induction of parthenocarpy in slicing and pickling germplasm. Two genotypes of gynoecious parthenocarpic cucumber, PPC-6 and DG-8, along with an MABC-derived gynoecious non-parthenocarpic line, IMPU-1, were evaluated in this study. The slicing and pickling cucumber genotypes PPC-6 and DG-8 were strongly parthenocarpic in nature and set fruit normally without pollination. Endogenous auxin and gibberellin were significantly higher in parthenocarpic than non-parthenocarpic genotypes, whereas the concentration of cytokinins varied among the genotypes at different developmental stages. However, the exogenous application of Zeatin and IAA + Zeatin was effective in inducing parthenocarpic fruit in IMPU-1. Expression analysis with important CK, AUX, and GA biosynthesis-related genes was conducted in IMPU-1, PPC-6, and DG-8. The expression of the CK synthase, IPT, IPT3, PaO, LOG1, LOG2, CYP735A1, and CYP735A2 was up-regulated in the parthenocarpic genotypes. Among the transcription factor response regulators (RRs), positive regulation of CSRR8/9b, CSRR8/9d, CSRR8/9e, and CSRR16/17 and negative feedback of the CK signalling genes, such as CsRR3/4a, CsRR3/4b, CsRR8/9a, and CsRR8/9c, were recorded in the parthenocarpic lines. Homeostasis between cytokinin biosynthesis and degradation genes such as CK oxidases (CKXs) and CK dehydrogenase resulted in a non-significant difference in the endogenous CK concentration in the parthenocarpic and non-parthenocarpic genotypes. In addition, up-regulation of the key auxin-inducing proteins and GA biosynthesis genes indicated their crucial role in the parthenocarpic fruit set of cucumber. This study establishes the critical role of the CKs, AUX, and GA regulatory networks and their cross-talk in determining parthenocarpy in slicing and pickling cucumber genotypes.
Not Available
 
Date 2022-10-25T06:09:50Z
2022-10-25T06:09:50Z
2022-08-26
 
Type Research Paper
 
Identifier Mandal NK, Kumari K, Kundu A, Arora A, Bhowmick PK, Iquebal MA, Jaiswal S, Behera TK, Munshi AD and Dey SS (2022), Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber. Front. Genet. 13:957360. doi: 10.3389/fgene.2022.957360
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/74729
 
Language English
 
Relation Not Available;
 
Publisher Not Available