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A rapid protocol to test genetic purity of pearl millet (Pennisetum glaucum) genotypes

Indian Agricultural Research Journals

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Title A rapid protocol to test genetic purity of pearl millet (Pennisetum glaucum) genotypes
 
Creator KUMAR, M B ARUN
SHERRY, R
VARIER, ANURADHA
 
Subject Esterases; Genetic purity testing; DDS testing
 
Description A new protocol has been standardized for analyzing seed esterases extracted from single seeds of pearl millet, which will be helpful in testing genetic purity of seed lot and for establishing the uniformity and stability of new cultivars during Distinctness, Uniformity and stability testing. Extraction ofcrude enzymes from single dry seed in 60Jll of 100 m  M Tris-Hel (pH 7.5) buffer and then leaving the contents for 4 hr at 4°C before centrifugation (at 14000 rpm for 15 minutes at 4°C) gave the similar electrophoretic banding pattern as that ofesterases extracted from 10 bulked seeds.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-01-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2507
 
Source The Indian Journal of Agricultural Sciences; Vol 76, No 2 (2006)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2507/641
 
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