Identification and characterization of differentially expressed novel miRNAs (21-24 nt) in a Macrophomina phaseolina resistant RIL line of jute (Corchorus capsularis L.)
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Title |
Identification and characterization of differentially expressed novel miRNAs (21-24 nt) in a Macrophomina phaseolina resistant RIL line of jute (Corchorus capsularis L.)
Not Available |
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Creator |
Dey, P., Biswas, C. and Karmakar, P. G
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Subject |
MicroRNA, Corchorus capsularis, Ubiquitin, NBS-LRR, ROS
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Description |
Not Available
A RIL population of jute developed by crossing one resistant accession CIM 036 and a susceptible variety JRC 412 was used to identify novel defence related miRNAs activated upon challenged inoculation with stem rot pathogen Macrophomina phaseolina. About a total of 15.7 million reads were generated from the resistant line with the read length of 1 × 50 bp. Here, we identified nine mature novel microRNAs which passed Minimum Free Energy (MFE Kcal/mol) criteria. Target site and secondary structure were predicted and most of them showed ubiquitination and selective autophagy activity with high expression value. Five novel miRNAs viz. Candidate_41, Candidate_9, Candidate_66, Candidate_65 and Candidate_8 had free energy less than −25 kcal/mol. Known microRNAs viz. miR-845b and miR-166 superfamily are abundantly expressed with high expression value. The sequence of jute miR-845b superfamily is identical to that of Arabidopsis thaliana except at 18th position, but unlike in A. thaliana it targets the coding sequence for the P-loop motif in the mRNA sequences for disease resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. In-silico analysis suggested that miR-845b and miR-166 superfamily provided NBS-LRR and ROS mediated defence and subsequently expression of novel microRNAs with selective autophagy activity enabled multi-layered defence cascade against M. phaseolina in jute. Not Available |
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Date |
2021-09-09T07:30:50Z
2021-09-09T07:30:50Z 2016-04-29 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/61909 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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