Deciphering the transcriptomic insight during organogenesis in castor (Ricinus communis L.), jatropha (Jatropha curcas L.) and sunflower (Helianthus annuus L.)
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Title |
Deciphering the transcriptomic insight during organogenesis in castor (Ricinus communis L.), jatropha (Jatropha curcas L.) and sunflower (Helianthus annuus L.)
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Creator |
Sai Sudha Puvvala
Tarakeswari Muddanuru Padmavathi AV Thangella Kumar Aniel O Navajeet Chakravartty Saurabh Gupta Vineeth Kodengil Vettath Krishna Mohan Ananta Venkata Sri Katta Sivarama Prasad Lekkala Boney Kuriakose Mulpuri Sujatha Vijay Bhasker Reddy Lachagari |
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Subject |
Auxins
Castor Callus DEGs RNA-seq Organogenesis |
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Description |
Not Available
Cultivation of the castor crop is hindered by various factors and one of the approaches for genetic improvement of the crop is through exploitation of biotechnological tools. Response of castor tissues to in vitro culture is poor which necessitated this study on understanding the molecular basis of organogenesis in cultured tissues of castor, through de novo transcriptome analysis and by comparing with jatropha and sunflower having good regeneration ability. Transcriptome profiling analysis was carried out with hypocotyl explants from castor, jatropha and cotyledons from sunflower cultured on MS media supplemented with different concentrations of hormones. Differentially expressed genes during dedifferentiation and organogenic differentiation stages of callus included components of auxin and cytokinin signaling, secondary metabolite synthesis, genes encoding transcription factors, receptor kinases and protein kinases. In castor, many genes involved in auxin biosynthesis and homeostasis like WAT1, vacuolar transporter genes, transcription factors like short root like protein were down-regulated while genes like DELLA were up-regulated accounting for regeneration recalcitrance. Validation of 62 DEGs through qRTPCR showed a consensus of 77.4% of the genes expressed. Overall study provides set of genes involved in the process of organogenesis in three oilseed crops which forms a basis for understanding and improving the efficiency of plant regeneration and genetic transformation in castor Not Available |
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Date |
2020-11-20T10:08:52Z
2020-11-20T10:08:52Z 2019-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/42502 |
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Language |
English
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Relation |
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Publisher |
Not Available
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