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Deciphering the transcriptomic insight during organogenesis in castor (Ricinus communis L.), jatropha (Jatropha curcas L.) and sunflower (Helianthus annuus L.)

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Title Deciphering the transcriptomic insight during organogenesis in castor (Ricinus communis L.), jatropha (Jatropha curcas L.) and sunflower (Helianthus annuus L.)
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Creator Sai Sudha Puvvala
Tarakeswari Muddanuru
Padmavathi AV Thangella
Kumar Aniel O
Navajeet Chakravartty
Saurabh Gupta
Vineeth Kodengil Vettath
Krishna Mohan Ananta Venkata Sri Katta
Sivarama Prasad Lekkala
Boney Kuriakose
Mulpuri Sujatha
Vijay Bhasker Reddy Lachagari
 
Subject Auxins 
Castor 
Callus 
DEGs
RNA-seq 
Organogenesis
 
Description Not Available
Cultivation of the castor crop is hindered by various factors and one of the approaches for genetic improvement of the crop is through exploitation of biotechnological tools. Response of castor tissues to in vitro culture is poor which necessitated this study on understanding the molecular basis of organogenesis in cultured tissues of castor, through de novo transcriptome analysis and by comparing with jatropha and sunflower having good regeneration ability. Transcriptome profiling analysis was carried out with hypocotyl explants from castor, jatropha and cotyledons from sunflower cultured on MS media supplemented with different concentrations of hormones. Differentially expressed genes during dedifferentiation and organogenic differentiation stages of callus included components of auxin and cytokinin signaling, secondary metabolite synthesis, genes encoding transcription factors, receptor kinases and protein kinases. In castor, many genes involved in auxin biosynthesis and homeostasis like WAT1, vacuolar transporter genes, transcription factors like short root like protein were down-regulated while genes like DELLA were up-regulated accounting for regeneration recalcitrance. Validation of 62 DEGs through qRTPCR showed a consensus of 77.4% of the genes expressed. Overall study provides set of genes involved in the process of organogenesis in three oilseed crops which forms a basis for understanding and improving the efficiency of plant regeneration and genetic transformation in castor
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Date 2020-11-20T10:08:52Z
2020-11-20T10:08:52Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/42502
 
Language English
 
Relation Not Available;
 
Publisher Not Available