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Development and evaluation of transgenic castor (Ricinus communis L.) expressing the insecticidal protein Cry1Aa of Bacillus thuringiensis against lepidopteran insect pests

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Title Development and evaluation of transgenic castor (Ricinus communis L.) expressing the insecticidal protein Cry1Aa of Bacillus thuringiensis against lepidopteran insect pests
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Creator Tarakeswari Muddanuru
Ananda Kumar Polumetla
Lakshminarayana Maddukuri
Sujatha Mulpuri
 
Subject Achaea janata
Bacillus thuringiensis
cry1Aa gene
Genetic transformation
Lepidoptera
Spodoptera litura
 
Description Not Available
Castor is an industrially valued non-edible oilseed crop. Susceptibility of the crop to foliage
feeders like Achaea janata (semilooper) and Spodoptera litura accounts for 30-50% of yield
losses. Owing to a lack of reliable sources of resistance to these lepidopteran pests, attempts
were made to develop transgenic events expressing the Bacillus thuringiensis (Bt) cry1Aa gene.
confirmed by polymerase chain reaction (PCR) and Southern hybridization analysis. Based on
segregation for a Mendelian ratio of 3:1, eight events (AMT-894, AMT-899, AK1304-PB-1,
AK1304-PB-4, AK1304-PB-785, AK1304-PB-830, AK1304-PB-837 and DTS-43) were
advanced. ELISA analysis detected protein from 0.16-2.76 ng/mg fresh leaf tissue across events
and in different generations. In laboratory insect bioassays, the mortality of S. litura and A.
janata ranged from 20-80% in different transgenic events and the weight reduction of surviving
larvae over the control larvae after 8 days of feeding was 28.4-87.2% in the case of S. litura and
27.9-78.1% for A. janata. In field bioassays, the event AMT-894 was most promising with 43% 34
of plants showing less than 25% leaf damage. As part of the regulatory requirement to check
the toxicity of the transgenic events to beneficial insects, larval bioassays against Samia cynthia
ricini (eri silkworm) using three transgenic events (AK1304-PB-1, AK1304-PB-4 and AMT-
894) showed a 20.2 to 78.5% reduction in weight
Not Available
 
Date 2020-11-20T10:00:07Z
2020-11-20T10:00:07Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/42497
 
Language English
 
Relation Not Available;
 
Publisher Not Available