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Rapid method of screening for drought stress tolerance in maize (Zea mays L.).

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Title Rapid method of screening for drought stress tolerance in maize (Zea mays L.).
Not Available
 
Creator Kumar B
Kumar K
Jat SL
Srivastava S
Tiwari T
Kumar S
Pradhan HR
Meenakshi
Kumar B
Chaturvedi G
Jha AK
Rakshit S
 
Subject Maize, drought tolerance, hydroponic solution, anti-oxidant genes, expression profiling
 
Description Not Available
Drought stress is the major production constraint in rainfed
maize. Screening for drought tolerance is severely affected
by the lack of a simple and reliable phenotyping technique.
The objective of this study was to standardize a simple
hydroponic based drought screening technique in maize.
In this context, one week old uniform seedlings of 55 inbreds
and 5 hybrids were transferred to hydroponic solution in
the glass house. The seedlings were allowed to acclimatize
for next one week in hydroponic solution. The drought
stress was imposed by removing seedlings from nutrient
solution and exposed to air for 6 and 4 hours daily for a
period of 5 and 4 consecutive days in hybrids and inbreds,
respectively. Data were recorded on all shoot and root
parameters, and based on stress symptoms, a drought
tolerance score was given to each genotype. The percent
deductions in shoot and root fresh weight from non-stress
to stress ranged from 11.7 to 84.4 and 2.1 to 77.5,
respectively. Six inbred lines, namely, DQL790-4, CML334,
CM140, CML422, CM125 and HKI488 and three hybrids
namely DMRH1306, DMRH1410 and PMH4 were found
drought tolerant. The effectiveness of this screening
technique was compared and confirmed using pots
screening as well as by expression profiling of key
antioxidant genes (Sod2, Sod4, Sod9 and Apx1) playing
role in drought stress tolerance. This phenotyping technique
is very short, low cost and simple which can be utilized in
preliminary drought screening for large set of maize
germplasm and mapping populations.
Not Available
 
Date 2020-12-09T09:13:22Z
2020-12-09T09:13:22Z
2020-02-11
 
Type Research Paper
 
Identifier Not Available
0019-5200
http://krishi.icar.gov.in/jspui/handle/123456789/43053
 
Language English
 
Relation Not Available;
 
Publisher the Indian Society of Genetics & Plant Breeding