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Cytomorphological and molecular characterization of inter-specific hybrid between cultivated sunflower and Helianthus argophyllus

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Title Cytomorphological and molecular characterization of inter-specific hybrid between cultivated sunflower and Helianthus argophyllus
Characterization of interspecific hybrid of sunflower
 
Creator Meena H.P.
Sujatha, M.
Pushpa, H.D.
Lal, J.J.
 
Subject Characters
Interspecific hybridization
Meiosis
Morphological
Pollination
Sunflower
 
Description Not Available
Aim: To study useful variability generated in an interspecific hybrid between cultivated sunflower inbred (ARM-243B) and silver leaf sunflower H. argophyllus (ARG-2126) through cytomorphological traits and hybrid confirmation by molecular markers.
Methodology: The present study on hybridization was undertaken using cultivated sunflower inbred (ARM-243B) and silver leaf sunflower H. argophyllus (ARG-2126). Thirty one morphological characters were recorded of cultivated sunflower (ARM-243B), H. argophyllus (ARG-2126) and interspecific hybrid. A total of 50 PMCs analysed for cytological observations. 62 SSR primers were used for molecular characterisation and hybridity confirmation in interspecific hybrid.
Results: Considerable variability was observed in interspecific hybrid, with common traits including branched stems, anthocyanin coloration in petiole and disk florets, leaf petioles, and stems etc. Cytological abnormalities and reduction in pollen fertility indicated that generated interspecific hybrid was true to type . Out of 62 SSR primers, 19 showed parental polymorphism; ORS-1021 showed 10 bp allelic variations in interspecific hybrid in a co-dominant manner and, hence, was used for hybridity confirmation. Further work on the confirmed interspecific hybrid with regard to generation advancement andselection of promising pre-breeding lines withagronomically superior traits is in progress.
Interpretation: The results of this investigation showed that interspecific hybridization between cultivated sunflower and H. argophyllus and the transfer of desirable traits into cultivated sunflower is possible.
Not Available
 
Date 2020-12-30T11:33:08Z
2020-12-30T11:33:08Z
2020-01-01
 
Type Research Paper
 
Identifier Meena H.P., Sujatha, M., Pushpa, H.D. and Lal, J.J. 2020. Cytomorphological and molecular characterization of inter-specific hybrid between cultivated sunflower and Helianthus argophyllus. Journal of Environmental Biology, 41: 66-72.
2394-0379
http://krishi.icar.gov.in/jspui/handle/123456789/44308
 
Language English
 
Relation Not Available;
 
Publisher Triveni Enterprises, Lucknow