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Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern&Coss.)

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Title Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern&Coss.)
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Creator Pushpa, H.D
Yadava, D.K
SujataVasudev
Vunu, V
Chandanabehera
Naveen Singh
 
Subject Indian mustard
oil quality
molecular markers
glucosimolates
 
Description Not Available
Six earlier reported markers closely linked to low glucosinolate QTLs of Brassica juncea, spread across ‘A’ genome (A2, A3 and A9) were validated in a recombinant inbred line (RIL) population of a cross between Pusa Mustard-21 (low erucic acid) and EC-597325 (double low) genotypes, to utilize them in marker-assisted selection (MAS). Of them, four markers viz., GER 1 amplified alleles of 650 bp and of 950 bp, GER 5 amplified 310 bp and 350 bp, At5gAJ67 amplified 500 bp and 450 bp and Myb28 amplified alleles of size 900 bp and 920 bp in EC597325 and Pusa Mustard-21, respectively and therefore differentiated low and high glucosinolate parents. These four polymorphic markers were then used to genotype the phenotyped RIL population consisting 608 plants. Marker-trait association was tested for goodness of fit using 2 test. Of the four markers, GER1 and GER5 showed higher phenotypic variance (R2 value) compared to the others, indicating their significance in determination of glucosinolates and prospects for use in MAS for development of Indian mustard genotypes with low glucosinolates content
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Date 2021-02-09T04:38:57Z
2021-02-09T04:38:57Z
2020-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/45287
 
Language English
 
Relation Not Available;
 
Publisher Not Available