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Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

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Title Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)
Not Available
 
Creator Singh, S., Singh, U.B., Malviya, D., Paul, S., Sahu, P.K., Trivedi, M., Paul, D. and Saxena, A.K.,
 
Subject seed bio-priming; microbial inoculant; anti-oxidative defense enzymes; Rhizoctonia solani; banded leaf and sheath blight; maize (Zea mays L.)
 
Description Not Available
Plant growth promoting rhizobacteria Pseudomonas aeruginosa strain MF-30 isolated from
maize rhizosphere was characterized for several plant growth stimulating attributes. The strain MF-30
was also evaluated for antifungal properties against Rhizoctonia solani causing banded leaf and sheath
blight in maize (Zea mays L.) under in vitro conditions and was found to have higher mycelial growth
suppression in the culture suspension (67.41%) followed by volatile organic compounds (62.66%)
and crude extract (51.20%) in a dual plate assay. The endophytic and epiphytic colonization ability
was tested using Green Fluorescent Protein (GFP)-tagging. Visualization through confocal scanning
laser microscope clearly indicated that strain MF-30 colonizes the root and foliar parts of the plants.
Further, the effects of seed bio-priming with P. aeruginosa MF-30 was evaluated in the induction and
bioaccumulation of defense-related biomolecules, enzymes, natural antioxidants, and other changes
in maize under pot trial. This not only provided protection from R. solani but also ensured growth
promotion under pathogenic stress conditions in maize. The maximum concentration of hydrogen
peroxide (H2O2) was reported in the root and shoot of the plants treated with R. solani alone (8.47 and
17.50 mmol mg−1 protein, respectively) compared to bioagent, P. aeruginosa MF-30 bio-primed plants
(3.49 and 7.50 mmol mg−1 protein, respectively). Effects on total soluble sugar content, total protein,
and total proline were also found to enhanced significantly due to inoculation of P. aeruginosa MF-30.
The activities of anti-oxidative defense enzymes phenylalanine ammonia lyase (PAL), ascorbate
peroxidase, peroxidase, superoxide dismutase, and catalase increased significantly in the plants
bio-primed with P. aeruginosa MF-30 and subsequent foliar spray of culture suspension of MF-30
compared to pathogen alone inoculated plants. qRT-PCR analysis revealed that seed bio-priming and
foliar application of P. aeruginosa MF-30 significantly increased the expression of PR-1 and PR-10 genes
with the simultaneous decrease in the disease severity and lesion length in the maize plants under
pathogenic stress conditions. A significant enhancement of shoot and root biomass was recorded in
MF-30 bio-primed plants as compared to untreated control (p < 0.05). Significant increase in plant
growth and antioxidant content, as well as decreased disease severity in the P. aeruginosa MF-30
bio-primed plants, suggested the possibility of an eco-friendly and economical means of achieving
antioxidants-rich, healthier maize plants.
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Date 2021-07-28T06:22:57Z
2021-07-28T06:22:57Z
2020-02-01
 
Type Research Paper
 
Identifier Singh, S., Singh, U.B., Malviya, D., Paul, S., Sahu, P.K., Trivedi, M., Paul, D. and Saxena, A.K., 2020. Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.). International Journal of Environmental Research and Public Health, 17(4), p.1396.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50530
 
Language English
 
Relation Not Available;
 
Publisher MDPI