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Biodegradation of Gossypol by Mixed Fungal Cultures in Minimal Medium

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Title Biodegradation of Gossypol by Mixed Fungal Cultures in Minimal Medium
Not Available
 
Creator V Mageshwaran
V Sharma
M Chinnkar
N Parvez
V Krishnan
 
Subject biodegradation, Candida tropicalis, gossypol, Pleurotus sajor-caju, Saccharomyces cerevisiae
 
Description Not Available
The fungal cultures, namely – Pleurotus sajor-caju MTCC 1806, Saccharomyces cerevisiae MTCC
6933 and Candida tropicalis MTCC 1406 and their combinations, C. tropicalis + S. cerevisiae, P. sajor-caju +
S. cerevisiae and C. tropicalis + P. sajor-caju were grown in minimal medium containing 100 ppm of gossypol
as the sole carbon and energy source. The culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju +
S. cerevisiae had low residual gossypol levels of 29 and 25 ppm, respectively. In the present study, we
attempted to isolate gossypol-degrading enzyme and biodegraded gossypol from the culture supernatants of
C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae. The specific activity of laccase in the purified
enzyme extracts of the C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae treated samples was 425
and 224 U/mg, respectively. In SDS-PAGE, the gossypol-degrading enzyme was revealed as 3 bands of
molecular weights ranging from 45 to 66 kDa. The characterization of biodegraded gossypol by FTIR analysis
showed a reduction in aldehydes (C-H) stretches in samples treated with fungi. Mass spectrometry analysis
revealed that the monoisotopic mass of the biodegraded gossypol was 474 g/mol.
National Agricultural Science Fund (NASF), New Delhi
 
Date 2021-07-28T10:22:07Z
2021-07-28T10:22:07Z
2018-01-01
 
Type Article
 
Identifier Mageshwaran V., Sharma V, M. Chinnkar, N. Parvez and V. Krishnan. 2018. Biodegradation of gossypol by mixed fungal cultures in minimal medium” Applied Biochemistry and Microbiology.54 (3): 301-308.le
ISSN 0003-6838
http://krishi.icar.gov.in/jspui/handle/123456789/50780
 
Language English
 
Relation Not Available;
 
Publisher Pleiades Publishing