Record Details

Restructuring the Cellular Responses: Connecting Microbial Intervention With Ecological Fitness and Adaptiveness to the Maize (Zea mays L.) Grown in Saline–Sodic Soil

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Restructuring the Cellular Responses: Connecting Microbial Intervention With Ecological Fitness and Adaptiveness to the Maize (Zea mays L.) Grown in Saline–Sodic Soil
Not Available
 
Creator Singh S, Singh UB, Trivdi M, Malviya D, Sahu PK, Roy M, Sharma PK, Singh HV, Manna MC and Saxena AK
 
Subject seed biopriming, rhizosphere microorganisms, Maize (Zea mays L), saline–sodic soil, antioxidant enzymes, salt tolerance, High-Affinity K+ Transporter, Sodium/hydrogen exchanger
 
Description Not Available
Salt stress hampers plant growth and development. It is now becoming one of
the most important threats to agricultural productivity. Rhizosphere microorganisms
play key roles in modulating cellular responses and enable plant tolerant to salt
stress, but the detailed mechanisms of how this occurs need in-depth investigation.
The present study elucidated that the microbe-mediated restructuring of the cellular
responses leads to ecological fitness and adaptiveness to the maize (Zea mays L.)
grown in saline–sodic soil. In the present study, effects of seed biopriming with
B. safensis MF-01, B. altitudinis MF-15, and B. velezensis MF-08 singly and in
consortium on different growth parameters were recorded. Soil biochemical and
enzymatic analyses were performed. The activity and gene expression of High-Affinity
K Transporter (ZmHKT-1), Sodium/Hydrogen exchanger 1 (zmNHX1), and antioxidant
enzymes (ZmAPX1.2, ZmBADH-1, ZmCAT, ZmMPK5, ZmMPK7, and ZmCPK11) were
studied. The expression of genes related to lateral root development (ZmHO-1, ZmGSL-
1, and ZmGSL-3) and root architecture were also carried out. Seeds bioprimed with
consortium of all three strains have been shown to confer increased seed germination
(23.34–26.31%) and vigor indices (vigor index I: 38.71–53.68% and vigor index II:
74.11–82.43%) as compared to untreated control plant grown in saline–sodic soil at
30 days of sowing. Results indicated that plants treated with consortium of three
strains induced early production of adventitious roots (tips: 4889.29, forks: 7951.57,
and crossings: 2296.45) in maize compared to plants primed with single strains and
untreated control (tips: 2019.25, forks: 3021.45, and crossings: 388.36), which was
further confirmed by assessing the transcript level of ZmHO-1 (7.20 folds), ZmGSL-
1 (4.50 folds), and ZmGSL-3 (12.00 folds) genes using the qPCR approach. The
uptake and translocation of Na, K, and Ca2 significantly varied in the plants
treated with bioagents alone or in consortium. qRT-PCR analysis also revealed that
the ZmHKT-1 and zmNHX1 expression levels varied significantly in the maize root upon inoculation and showed a 6- to 11-fold increase in the plants bioprimed with all the three
strains in combination. Further, the activity and gene expression levels of antioxidant
enzymes were significantly higher in the leaves of maize subjected seed biopriming with
bioagents individually or in combination (3.50- to 12.00-fold). Our research indicated
that ZmHKT-1 and zmNHX1 expression could effectively enhance salt tolerance by
maintaining an optimal Na/K balance and increasing the antioxidant activity that
keeps reactive oxygen species at a low accumulation level. Interestingly, up-regulation of
ZmHKT-1, NHX1, ZmHO-1, ZmGSL-1, and ZmGSL-3 and genes encoding antioxidants
regulates the cellular responses that could effectively enhance the adaptiveness and
ultimately leads to better plant growth and grain production in the maize crop grown in
saline–sodic soil.
Not Available
 
Date 2021-07-28T11:24:12Z
2021-07-28T11:24:12Z
2021-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50839
 
Language English
 
Relation Not Available;
 
Publisher Frontiers