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Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes

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Title Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes
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Creator Rajavat AS, Rai S, Pandiyan K, Kushwaha P., Choudhary P, Kumar M, Chakdar H, Singh A, Karthikeyan N, Bagul SY, Agnihotri A, Saxena AK
 
Subject lignocellulolytic enzymes,Pleurotus florida, solid‐state fermentation, spent mushroom substrate
 
Description Not Available
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57–74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid‐state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A‐01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C‐08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2‐, 4.5‐, 4.1‐, and 5.0‐fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost‐effective substrate for production of cellulolytic enzymes from T. longibrachiatum A‐01 and A. aculeatus C‐08.
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Date 2021-09-06T17:03:24Z
2021-09-06T17:03:24Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/61466
 
Language English
 
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Publisher Not Available