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noxB-based marker for Alternaria spp.: a new diagnostic marker for specific and early detection in crop plants

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Title noxB-based marker for Alternaria spp.: a new diagnostic marker for specific and early detection in crop plants
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Creator Chakdar H, Goswami SK, Singh E, Choudhary P, Yadav J, Kashyap PL, Srivastava AK and Saxena AK
 
Subject Alternaria; Diagnostic marker; Molecular detection noxB; PCR Real-time PCR
 
Description Not Available
Alternaria species are a major plant pathogen and their precise detection and identification is crucial for effective management. In the present study, a polymerase chain reaction (PCR)-based diagnostic technique has been developed for detection of Alternaria species. Four primers were designed for four genes viz. noxB, AMK1, AKT3 and NIK1. In gradient PCR, only the primer sets for noxB gene showed specific amplicon of ~ 200 bp in all the isolates of Alternaria, while no amplification was observed in related fungal species such as Ulocladium botrytis, Ulocladium consortiale, Stemphylium vesicarium, Cochliobolus tuberculatus, Curvularia prasadii, and Bipolaris sorokiniana. The noxB primer set was used as diagnostic marker to discriminate and diagnose Alternaria species in nine different crop plants. Real-time assay revealed that the primer set was able to detect Alternaria noxB genes in leaves with no characteristic visible symptoms. Through real-time PCR, the noxB gene of Alternaria could be detected even in 0.5 ng of host DNA. This is the first report of noxB gene for molecular detection of Alternaria spp.
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Date 2021-09-06T16:59:32Z
2021-09-06T16:59:32Z
2019-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/61448
 
Language English
 
Relation Not Available;
 
Publisher Springer