Record Details

A modified plate assay for rapid screening of Potassium-Solubilizing Bacteria.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title A modified plate assay for rapid screening of Potassium-Solubilizing Bacteria.
Not Available
 
Creator Rajawat MVS, Singh S, Tyagi SP, Saxena AK
 
Subject bromothymol blue (BTB); Aleksandrov medium; halo zone formation; indicator dye; K solubilizers; microorganisms
 
Description Not Available
The utility of microorganisms for solubilizing the unavailable forms of potassium (K) from soil has led to renewed interest in fabrication of rapid and sensitive plate assays for their isolation and screening. The present study developed a modified plate assay and compared it with previously reported methods for the isolation and screening of K-solubilizing bacteria. The newly developed plate assay is based on improved visualization of halo zone formation around the colonies on agar plates, through inclusion of an acid-base indicator dye, bromothymol blue (BTB), to modify the previously reported Aleksandrov medium. The halo zone exhibited a significant correlation (R = 0.939) with K released in liquid medium. The visualization of potential K solubilizers was improved using this method, which would help in detection of weak/non-acid producers based on secretion of organic acids in the medium. Organic acids in plate diffuse radially and form halo zones in response to reaction with the acid-base indicator dye BTB. Furthermore, K solubilization on plates with this method can be observed within 48–72 h, against the incubation time of 4–5 d needed in the earlier method. Therefore, the newly developed protocol for the plate assay was time saving, more sensitive, and beneficial in comparison to the previously reported Aleksandrov plate assay.
Not Available
 
Date 2021-09-22T09:27:23Z
2021-09-22T09:27:23Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64579
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER