Record Details

Culturable diversity and functional annotation of psychrotrophic bacteria from cold desert of Leh Ladakh (India)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Culturable diversity and functional annotation of psychrotrophic bacteria from cold desert of Leh Ladakh (India)
Not Available
 
Creator Yadav AN, Sachan SG, Verma P, Taygi SP, Kaushik R, Saxena AK
 
Subject Cold desert Diversity Leh Ladakh PGPB Psychrotrophic bacteria 16S rRNA gene
 
Description Not Available
o study culturable bacterial diversity undersubzero temperature conditions and their possible functionalannotation, soil and water samples from Leh Ladakh regionwere analysed. Ten different nutrient combinations wereused to isolate the maximum possible culturable morpho-types. A total of 325 bacterial isolates were characterizedemploying 16S rDNA-Amplified Ribosomal DNA Restric-tion Analysis with three restriction endonucleasesAluI,MspI andHaeIII, which led to formation of 23–40 groups forthe different sites at 75 % similarity index, adding up to 175groups. Phylogenetic analysis based on 16S rRNA genesequencing led to the identification of 175 bacteria, groupedin four phyla, Firmicutes (54 %), Proteobacteria (28 %),Actinobacteria (16 %) and Bacteroidetes (3 %), and inclu-ded 29 different genera with 57 distinct species. Overall39 % of the total morphotypes belonged to theBacillusandBacillusderived genera (BBDG) followed byPseudomonas(14 %),Arthrobacter(9 %),Exiguobacterium(8 %),Ali-shewanella(4 %),Brachybacterium, Providencia, Plano-coccus(3 %),Janthinobacterium,Sphingobacterium,Kocuria(2 %) andAurantimonas, Citricoccus, Cellulosi-microbium, Brevundimonas, Desemzia, Flavobacterium,Klebsiella, Paracoccus, Psychrobacter, Sporosarcina,Staphylococcus, Sinobaca, Stenotrophomonas, Sanguib-acter, Vibrio(1 %). The representative isolates from eachcluster were screened for their plant growth promotingcharacteristics at low temperature (5–15°C). Variationswere observed among strains for production of ammonia,hydrogen cyanide, indole-3-acetic acid and siderophore,solubilisation of phosphate, 1-aminocyclopropane-1-car-boxylate deaminase activity and biocontrol activity againstRhizoctonia solaniandMacrophomina phaseolina.Coldadapted microbes may have application as inoculants andbiocontrol agents in crops growing at high altitudes undercold climate condition.
Not Available
 
Date 2021-10-14T10:53:42Z
2021-10-14T10:53:42Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/65739
 
Language English
 
Relation Not Available;
 
Publisher Not Available