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Deciphering the diversity of culturable thermotolerant bacteria from Manikaran hot springs

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Title Deciphering the diversity of culturable thermotolerant bacteria from Manikaran hot springs
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Creator Kumar M, Yadav A N, Tiwari R, Prasanna R and Saxena A. K.
 
Subject Culturable bacteria 16S rRNA gene ARDRA Sequencing BIOLOG analysis Enzymes
 
Description Not Available
The aim of this study was to analyze and characterize the diversity of culturable thermotolerant bacteria in Manikaran hot springs. A total of 235 isolates were obtained employing different media, and screened for temperature tolerance (40 °C–70 °C). A set of 85 isolates tolerant to 45 °C or above were placed in 42 phylogenetic clusters after amplified ribosomal DNA restriction analysis (16S rRNA-ARDRA). Sequencing of the 16S rRNA gene of 42 representative isolates followed by BLAST search revealed that the majority of isolates belonged to Firmicutes, followed by equal representation of Actinobacteria and Proteobacteria. Screening of representative isolates (42 ARDRA phylotypes) for amylase activity revealed that 26 % of the isolates were positive, while 45 % exhibited protease activity, among which one amylase and six protease producers were tolerant up to 70 °C. BIOLOG-based identification of 13 isolates exhibiting temperature tolerance up to 70 °C, using carbon utilization patterns and sensitivity to chemicals, revealed a high degree of correlation with identification based on 16S rRNA gene sequencing for all isolates, except one (M48). These promising isolates showing a range of useful metabolic attributes demand to be explored further for industrial and agricultural applications.
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Date 2021-10-18T11:02:23Z
2021-10-18T11:02:23Z
2013-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/65840
 
Language English
 
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