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Antifungal characteristics of a fluorescent Pseudomonas strain involved in the biological control of Rhizoctonia solani

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Title Antifungal characteristics of a fluorescent Pseudomonas strain involved in the biological control of Rhizoctonia solani
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Creator Pal, K.K., Tilak, K.V.B.R Saxena, A.K., Rinku Dey and Singh, C.S.,
 
Subject antibiotics; fluorescent pigment biocontrol; Tn5::lacZ mutagenesis
 
Description Not Available
A plant growth-promoting isolate of a fluorescent Pseudomonas spp. EM85 was found strongly antagonistic to Rhizoctonia solani, a causal agent of damping-off of cotton. The isolate produced HCN (HCN+), siderophore (Sid+), fluorescent pigments (Flu+) and antifungal antibiotics (Afa+). Tn5::lacZ mutagenesis of isolate EM85 resulted in the production of a series of mutants with altered production of HCN, siderophore, fluorescent pigments and antifungal antibiotics. Characterisation of these mutants revealed that the fluorescent pigment produced in PDA and the siderophore produced in CAS agar were not the same. Afa− and Flu− mutants had a smaller inhibition zone when grown with Rhizoctonia solani than the EM85 wild type. Sid− and HCN− mutants failed to inhibit the pathogen in vitro. In a pot experiment, mutants deficient in HCN and siderophore production could suppress the dampingoff disease by 52%. However, mutants deficient in fluorescent pigments and antifungal antibiotics failed to reduce the disease severity. Treatments with mutants that produced enhanced amounts of fluorescent pigments and antibiotics compared with EM85 wild type, exhibited an increase in biocontrol efficiency. Monitoring of the mutants in the rhizosphere using the lacZ marker showed identical proliferation of mutants and wild type. Purified antifungal compounds (fluorescent pigment and antibiotic) also inhibited the fungus appreciably in a TLC bioassay. Thus, the results indicate that fluorescent pigment and antifungal antibiotic of the fluorescent Pseudomonas spp. EM85 might be involved in the biological suppression of Rhizoctonia-induced damping-off of cotton.
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Date 2021-11-01T10:22:21Z
2021-11-01T10:22:21Z
2002-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/66735
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER