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Selection of Tn5 :: lacZ mutants isogenic to wild type Azospirillum brasilense strains capable of growing at suboptimal temperature

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Title Selection of Tn5 :: lacZ mutants isogenic to wild type Azospirillum brasilense strains capable of growing at suboptimal temperature
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Creator Kaushik, R., Saxena, A.K. and Tilak, K.V.B.R.
 
Subject Azospirillum root colonization sub-optimal temperature Tn5-lacZ
 
Description Not Available
Azospirillum brasilense strains, CDJA and A40, capable of growing at sub-optimal temperature were tagged with stable chromogenic marker Tn5-lacZ. Mutants were screened for plant growth promoting activities at 20, 22, 25, 30 and 37 °C. Mutants MC48 and MA3 were found to fix nitrogen upto 85% and produced indole acetic acid (IAA) and siderophore in isogenic manner to their respective wild type strains, CDJA and A40, at sub-optimal temperatures. Co-inoculation of mutants with their respective parent (1:1 ratio) to the wheat revealed that colonization potential of the mutants was affected greatly. Tn5-lacZ tagged mutants MC48 and MA3 were found isogenic to their respective wild type Azospirillum strain, with regards to plant growth promoting activities and root colonization ability.
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Date 2021-11-01T10:24:07Z
2021-11-01T10:24:07Z
2000-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/66742
 
Language English
 
Relation Not Available;
 
Publisher Springer