Selection of Tn5 :: lacZ mutants isogenic to wild type Azospirillum brasilense strains capable of growing at suboptimal temperature
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Title |
Selection of Tn5 :: lacZ mutants isogenic to wild type Azospirillum brasilense strains capable of growing at suboptimal temperature
Not Available |
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Creator |
Kaushik, R., Saxena, A.K. and Tilak, K.V.B.R.
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Subject |
Azospirillum root colonization sub-optimal temperature Tn5-lacZ
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Description |
Not Available
Azospirillum brasilense strains, CDJA and A40, capable of growing at sub-optimal temperature were tagged with stable chromogenic marker Tn5-lacZ. Mutants were screened for plant growth promoting activities at 20, 22, 25, 30 and 37 °C. Mutants MC48 and MA3 were found to fix nitrogen upto 85% and produced indole acetic acid (IAA) and siderophore in isogenic manner to their respective wild type strains, CDJA and A40, at sub-optimal temperatures. Co-inoculation of mutants with their respective parent (1:1 ratio) to the wheat revealed that colonization potential of the mutants was affected greatly. Tn5-lacZ tagged mutants MC48 and MA3 were found isogenic to their respective wild type Azospirillum strain, with regards to plant growth promoting activities and root colonization ability. Not Available |
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Date |
2021-11-01T10:24:07Z
2021-11-01T10:24:07Z 2000-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/66742 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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