Identification and evolutionary analysis of polycistronic miRNA clusters in domesticated and wild wheat
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Title |
Identification and evolutionary analysis of polycistronic miRNA clusters in domesticated and wild wheat
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Creator |
Singh, Amit Kumar
Singh, Nidhi Kumar, Sundeep Kumari, Jyoti Singh, Rakesh Gaba, Sonam Yadav, Mahesh C. Grover, Monendra Chaurasia, Shiksha Kumar, Rajesh |
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Subject |
miRNA
Wheat genome Evolution Polycistronic miRNA |
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Description |
The authors thank Director ICAR-NBPGR, New Delhi for providing lab facilities to carry out this work. This work was supported by the Indian Council of Agriculture Research-National Innovations in Climate Resilient Agriculture project scheme (Grant number 1006607).
MicroRNAs are similar to 22 nucleotide long non-coding RNAs that regulate gene expression at posttranscriptional level. Genome-wide analysis was performed to identify polycistronic miRNAs from wheat. Total 89 polycistronic miRNAs were identified in bread wheat which were distributed on three component sub-genomes (A = 26, B = 33 and D = 30). Except some, most of the identified polycistronic miRNAs were also present in other cultivated and wild wheat species. Expression of 11 identified polycistronic miRNAs could be validated using previously assembled transcriptomes, RNA-seq/s-RNA seq data of cultivated and wild wheats and RT-PCR. Polycistronic miRNAs orthologs were also localized on rice and Brachypodium genomes. As a case study, we also analyzed molecular evolution of miR395 family polycistrons in wheat. Both tandem and segmental duplications contributed to expansion of miR395 family polycistrons. Our findings provide a comprehensive view on wheat polycitronic miRNAs that will enable their in-depth functional analysis in the future. Indian Council of Agriculture Research-National Innovations in Climate Resilient Agriculture project scheme [1006607] |
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Date |
2020-08-01T03:50:44Z
2020-08-01T03:50:44Z 2020-05 |
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Type |
Article
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Identifier |
Singh, Amit Kumar; Singh, Nidhi; Kumar, Sundeep; Kumari, Jyoti; Singh, Rakesh; Gaba, Sonam; Yadav, Mahesh C.; Grover, Monendra; Chaurasia, Shiksha; Kumar, Rajesh. Identification and evolutionary analysis of polycistronic miRNA clusters in domesticated and wild wheat, GENOMICS, 112 (3), 2334-2348. doi:10.1016/j.ygeno.2020.01.005
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/38862 |
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Language |
English
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Relation |
Not Available
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Publisher |
GENOMICS, ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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