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Expression of Cry2Aa, a Bacillus thuringiensis insecticidal protein in transgenic pigeon pea confers resistance to gram pod borer, Helicoverpa armigera.

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Title Expression of Cry2Aa, a Bacillus thuringiensis insecticidal protein in transgenic pigeon pea confers resistance to gram pod borer, Helicoverpa armigera.
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Creator Not Available20. Singh, S., Kumar, N. R., Maniraj, R., Lakshmikanth, R., Rao, K. Y. S., Muralimohan, N., ... Pattanayak, D and Rohini Sreevathsa
 
Subject Pigeonpea, cry2Aa, transgenics, pod borer, Bt ICPs
 
Description Not Available
Pigeon pea is an important legume infested by a plethora of insect pests amongst which gram pod
borer Helicoverpa armigera is very prominent. Imparting resistance to this insect herbivore is of global
importance in attaining food security. Expression of insecticidal crystal proteins (ICP) in diverse
crops has led to increased resistance to several pests. We report in this paper, expression of Cry2Aa in
transgenic pigeon pea and its efectiveness towards H. armigera by employing Agrobacterium-mediated
in planta transformation approach. Approximately 0.8% of T1 generation plants were identifed as
putative transformants based on screening in the presence of 70 ppm kanamycin as the selection agent.
Promising events were further recognized in advanced generations based on integration, expression
and bioefcacy of the transgenes. Seven T3 lines (11.8% of the selected T1 events) were categorized
as superior as these events demonstrated 80–100% mortality of the challenged larvae and improved
ability to prevent damage caused by the larvae. The selected transgenic plants accumulated Cry2Aa in
the range of 25–80µg/g FW. The transgenic events developed in the study can be used in pigeon pea
improvement programmes for pod borer resistance.
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Date 2020-08-22T04:13:24Z
2020-08-22T04:13:24Z
2018-06-11
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/39942
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature