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Comparative transcriptome analysis of pigeonpea, Cajanus cajan (L.) and one of its wild relatives Cajanus platycarpus (Benth.) Maesen.

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Title Comparative transcriptome analysis of pigeonpea, Cajanus cajan (L.) and one of its wild relatives Cajanus platycarpus (Benth.) Maesen.
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Creator Rathinam, Maniraj, Pragya Mishra, Madavan Vasudevan, Roli Budhwar, Ajay Mahato, A. Lakshmi Prabha, Nagendra Kumar Singh, Uma Rao, and Rohini Sreevathsa.
 
Subject Pigeonpea, pod borer, wild relative
 
Description Not Available
Pigeonpea is a major source of dietary protein to the vegetarian population of the Indian subcontinent.
Crop improvement to mitigate biotic and abiotic stresses for realization of its potential
yield and bridging yield gap is the need of the hour. Availability of limited genomic resources in
the cultivated germplasm, however, is a serious bottleneck towards successful molecular breeding
for the development of superior genotypes in pigeonpea. In view of this, improvement of
pigeonpea can be attempted through transgenesis or by exploiting genetic resources from its
wild relatives. Pigeonpea wild relatives are known to be bestowed with agronomic traits of importance;
discovery and deployment of genes from them can provide a lucrative option for crop
improvement. Understanding molecular signatures of wild relatives would not only provide information
about the mechanism behind desired traits but also enable us to extrapolate the information
to cultivated pigeonpea. The present study deals with the characterization of leaf
transcriptomes of Cajanus cajan and one of its wild relatives, Cajanus platycarpus. Illumina
sequencing revealed 0.11 million transcripts in both the species with an annotation of 0.09 million
(82%) transcripts using BLASTX. Comparative transcriptome analyses on the whole,
divulged cues about the wild relative being vigilant and agile. Gene ontology and Mapman analysis
depicted higher number of transcripts in the wild relative pertaining to signaling, transcription
factors and stress responsive genes. Further, networking between the differentially expressed
MapMan bins demonstrated conspicuous interactions between different bins through 535
nodes (512 Genes and 23 Pathways) and 1857 edges. The authenticity of RNA-seq analysis
was confirmed by qRT-PCR. The information emanating from this study can provide valuable
information and resource for future translational research including genome editing to alleviate
varied stresses. Further, this learning can be a platform for in-depth investigations to decipher
molecular mechanisms for mitigation of various stresses in the wild relative.
Not Available
 
Date 2020-08-22T04:16:49Z
2020-08-22T04:16:49Z
2019-07-03
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/39945
 
Language English
 
Relation Not Available;
 
Publisher PLOS