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Comparative transcriptome analyses provide novel insights into the differential response of Pigeonpea (Cajanus cajan L.) and its wild relative (Cajanus platycarpus (Benth.) Maesen) to herbivory by Helicoverpa armigera (Hübner).

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Title Comparative transcriptome analyses provide novel insights into the differential response of Pigeonpea (Cajanus cajan L.) and its wild relative (Cajanus platycarpus (Benth.) Maesen) to herbivory by Helicoverpa armigera (Hübner).
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Creator Rathinam, Maniraj, Pragya Mishra, Ajay Kumar Mahato, Nagendra Kumar Singh, Uma Rao, and Rohini Sreevathsa
 
Subject Pigeonpea, pod borer, wild relative, dynamic transcriptome
 
Description Not Available
Devastation by pod borer, Helicoverpa armigera is one of the major factors for stagnated productivity in Pigeonpea.
Despite possessing a multitude of desirable traits including pod borer resistance, wild relatives of Cajanus spp. have
remained under-utilized due to linkage drag and cross-incompatibility. Discovery and deployment of genes from them can
provide means to tackle key pests like H. armigera. Transcriptomic differences between Cajanus platycarpus and Cajanus
cajan during different time points (0, 18, 38, 96 h) of pod borer infestation were elucidated in this study. For the first ever
time, we demonstrated captivating variations in their response; C. platycarpus apparently being reasonably agile with
effectual transcriptomic reprogramming to deter the insect. Deeper insights into the differential response were obtained by
identification of significant GO-terms related to herbivory followed by combined KEGG and ontology analyses. C. platycarpus
portrayed a multilevel response with cardinal involvement of SAR, redox homeostasis and reconfiguration of primary
metabolites leading to a comprehensive defense response. The credibility of RNA-seq analyses was ascertained by transient
expression of selected putative insect resistance genes from C. platycarpus viz., chitinase (CHI4), Alpha-amylase/subtilisin
inhibitor (IAAS) and Flavonoid 3_5 hydroxylase (C75A1) in Nicotiana benthamiana followed by efficacy analysis against
H. armigera. qPCR validated results of the study provided innovative insights and useful leads for development of durable
pod borer resistance.
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Date 2020-08-22T04:16:22Z
2020-08-22T04:16:22Z
2019-07-04
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/39944
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature