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Allele mining for a drought responsive gene DRO1 determining root growth angle in donors of drought tolerance in rice (Oryza sativa L.)

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Title Allele mining for a drought responsive gene DRO1 determining root growth angle in donors of drought tolerance in rice (Oryza sativa L.)
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Creator Bablee Kumari Singh, M. K. Ramkumar, Monika Dalal, Archana Singh, Amolkumar U. Solanke, Nagendra K. Singh and Amitha Mithra Sevanthi
 
Subject Rice, Drought stress, Root growth angle, DRO1, Allele mining
 
Description Not Available
Deeper Rooting 1 (DRO1) gene identified from a major QTL on chromosome 9 increases the root growth angle (RGA) and thus facilitates survival under drought and hence is an excellent candidate for rice improvement. Twenty-four major Indian upland and lowland genotypes including the ‘yield under drought’ (DTY) QTL donors were subjected to allele mining of DRO1 (3058 bp) using four pairs of overlapping primers. A total of 216 and 52 SNPs were identified across all genotypes in the gene and coding region (756 bp) respectively with transversions 3.6 fold more common than transitions in the gene and 2.5 times in the CDS. In 251 amino acid long protein, substitutions were found in 19 positions, wherein change in position 92 was the most frequent. Based on allele mining, the 24 genotypes can be classified into 16 primary structure variants ranging from complete functional allele (Satti, IR36 and DTY 3.1 donor, IR81896-B-B-195) to truncated non-functional alleles in PMK2, IR64, IR20 and Swarna. All the DTY donors, other than IR81896-B-B-195, and most of the upland drought tolerant cultivars (Nagina 22, Vandana and Dhagaddeshi) had accumulated 6–19 SNPs and 4–8 amino acid substitutions resulting in substantial differences in their protein structure. The expression analysis revealed that all the genotypes showed upregulation under drought stress though the degree of upregulation varied among genotypes. The information on structural variations in DRO1 gene will be very useful for the breeders, especially in the light of recent breeding programmes on improving drought tolerance using several DTY donors and upland accessions.
 
Date 2021-07-08T08:55:35Z
2021-07-08T08:55:35Z
2021-02-26
 
Type Research Paper
 
Identifier Singh, B.K., Ramkumar, M.K., Dalal, M. et al. Allele mining for a drought responsive gene DRO1 determining root growth angle in donors of drought tolerance in rice (Oryza sativa L.). Physiol Mol Biol Plants 27, 523–534 (2021). https://doi.org/10.1007/s12298-021-00950-2
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http://krishi.icar.gov.in/jspui/handle/123456789/47565
 
Language English
 
Relation Not Available;
 
Publisher Springer