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Host-mediated RNAi of a Notch-like receptor gene in Meloidogyne incognita induces nematode resistance.

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Title Host-mediated RNAi of a Notch-like receptor gene in Meloidogyne incognita induces nematode resistance.
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Creator Kohli D, Chidambaranathan, P, Tej Kumar JP, Singh AK, Kumar A, Subramaniam K, Srinivasan R, Bharadvaja, N and Jain PK
 
Subject HD RNAi, nematode resistance, gene
 
Description Not Available
GLP-1 (abnormal germline proliferation) is a Notch-like receptor protein that plays an essential
role in pharyngeal development. In this study, an orthologue of Caenorhabditis elegans
glp-1 was identified in Meloidogyne incognita. A computational analysis revealed that the
orthologue contained almost all the domains present in the C. elegans gene: specifically, the
LIN-12/Notch repeat, the ankyrin repeat, a transmembrane domain and different ligand-binding
motifs were present in orthologue, but the epidermal growth factor-like motif was not
observed. An expression analysis showed differential expression of glp-1 throughout the life
cycle of M. incognita, with relatively higher expression in the egg stage. To evaluate the silencing
efficacy of Mi-glp-1, transgenic Arabidopsis plants carrying double-stranded RNA constructs
of glp-1 were generated, and infection of these plants with M. incognita resulted in
a 47–50% reduction in the numbers of galls, females and egg masses. Females obtained
from the transgenic RNAi lines exhibited 40–60% reductions in the transcript levels of the
targeted glp-1 gene compared with females isolated from the control plants. Second-generation
juveniles (J2s), which were descendants of the infected females from the transgenic
lines, showed aberrant phenotypes. These J2s exhibited a significant decrease in the overall
distance from the stylet to the metacorpus region, and this effect was accompanied by disruption
around the metacorporeal bulb of the pharynx. The present study suggests a role for this
gene in organ (pharynx) development during embryogenesis in M. incognita and its potential
use as a target in the management of nematode infestations in plants.
Not Available
 
Date 2021-07-09T12:00:38Z
2021-07-09T12:00:38Z
2018-03-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/47611
 
Language English
 
Relation Not Available;
 
Publisher Not Available