Record Details

Genome-wide identification of Ran GTPase family genes from wheat (T. aestivum) and their expression profile during developmental stages and abiotic stress conditions

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Genome-wide identification of Ran GTPase family genes from wheat (T. aestivum) and their expression profile during developmental stages and abiotic stress conditions
Not Available
 
Creator Choudhury S, Mansi, Muthusamy SK, Padaria JC, Dalal M
 
Subject Ran GTPase, wheat
 
Description Not Available
Maintenance of growth is important for sustaining yield under stress conditions. Hence, identification of genes involved in cell
division and growth under abiotic stress is utmost important. Ras-related nuclear protein (Ran) is a small GTPase required for
nucleocytoplasmic transport,mitotic progression, and nuclear envelope assembly in plants. In the present study, two Ran GTPase
genes TaRAN1 and TaRAN2 were identified though genome-wide analysis in wheat (T. aestivum). Comparative analysis of Ran
GTPases from wheat, barley, rice, maize, sorghum, and Arabidopsis revealed similar gene structure within phylogenetic clades
and highly conserved protein structure. Expression analysis from expVIP platform showed ubiquitous expression of TaRAN
genes across tissues and developmental stages. Under biotic and abiotic stresses, TaRAN1 expression was largely unaltered,
while TaRAN2 showed stress specific response. In qRT-PCR analysis, TaRAN1 showed significantly higher expression as
compared to TaRAN2 in shoot and root at seedling, vegetative, and reproductive stages. During progressive drought stress,
TaRAN1 and TaRAN2 expression increase during early stress and restored to control level expression at higher stress levels in
shoot. The steady-state level of transcripts was maintained to that of control in roots under drought stress. Under cold stress,
expression of both the TaRAN genes decreased significantly at 3 h and became similar to control at 6 h in shoots, while salt stress
significantly reduced the expression of TaRAN genes in shoots. The analysis suggests differential regulation of TaRAN genes
under developmental stages and abiotic stresses. Delineating the molecular functions of Ran GTPases will help unravel the
mechanism of stress induced growth inhibition in wheat.
ICAR_NIPB
 
Date 2021-07-28T06:27:57Z
2021-07-28T06:27:57Z
2021-01-01
 
Type Research Paper
 
Identifier Choudhury S, Mansi, Muthusamy SK, Padaria JC, Dalal M. Genome-wide identification of Ran GTPase family genes from wheat (T. aestivum) and their expression profile during developmental stages and abiotic stress conditions. Funct Integr Genomics. 2021 Mar;21(2):239-250. doi:
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50560
 
Language English
 
Relation Not Available;
 
Publisher springer