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Cultivar specific response of callus induction and plant regeneration from mature embryos in different elite Indian wheat

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Title Cultivar specific response of callus induction and plant regeneration from mature embryos in different elite Indian wheat
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Creator Not Available
Padaria Jasdeep, Tarafdar Avijit, Sharma Varsha, Vishwakarma Harinder, Singh G.P. and Singh Sanjay
 
Subject Bread wheat, in vitro culture, mature embryos, callus, regeneration
 
Description Not Available
To develop a successful cultivar-independent in vitro plant regeneration protocol in wheat, mature embryos
of nine Indian elite wheat (Triticum aestivum) cultivars were taken to establish a reliable effective,
reproducible callus culture and plant regeneration procedure. Two different auxins, naphtalenacetic acid
(NAA) and dichlorophenoxyacetic acid (2,4-D) and wo different cytokinins, 6-Benzylaminopurine (BAP)
and kinetin (Kn) were assessed in several combinations for their effect on callus induction and plant
regeneration from mature embryos in nine elite Indian wheat cultivars. The cultivar-dependent substantial
variances were noted in per cent of regeneration response. The callus induction was assessed in terms of
size of callus induced in seven days of initiation. Induction of callus did happen in all cultivars on all
media combinations tried, though there was difference in size of callus induced in different cultivars cultured
with different combination of growth regulators. However, the induced callus, irrespective of cultivar
when nurtured on MS media supplemented with 2,4-D (4.0 mg/L) and NAA (1.0 mg/L or 2.0 mg/L) showed
prominent callus growth. There were significant differences among the nine cultivars in regeneration of
shoots from embryonal callus on the various shooting media used. In spite of cultivar dependent response, the
MS media supplemented with kn (2.0 mg/L), NAA (0.5mg/L) and BAP (0.5 mg/L) was identified as the best
media for shoot regeneration for some of the elite Indian cultivars.
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Date 2021-07-29T08:38:21Z
2021-07-29T08:38:21Z
2019-02-10
 
Type Research Paper
 
Identifier 06
0973-6263 (print), 2278-4535
http://krishi.icar.gov.in/jspui/handle/123456789/51013
 
Language English
 
Relation Not Available;
 
Publisher Research Journal Biotechnology