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Transcriptome Analysis Reveals Important Candidate Genes Related to Nutrient Reservoir, Carbohydrate Metabolism, and Defence Proteins during Grain Development of Hexaploid Bread Wheat and Its Diploid Progenitors

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Title Transcriptome Analysis Reveals Important Candidate Genes Related to Nutrient Reservoir, Carbohydrate Metabolism, and Defence Proteins during Grain Development of Hexaploid Bread Wheat and Its Diploid Progenitors
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Creator Megha Kaushik
Shubham Rai
Sureshkumar Venkadesan
Subodh Kumar Sinha
Sumedha Mohan
Pranab Kumar Mandal
 
Subject Molecular biology and biotechnology
 
Description Not Available
Wheat grain development after anthesis is an important biological process, in which major
components of seeds are synthesised, and these components are further required for germination
and seed vigour. We have made a comparative RNA-Seq analysis between hexaploid wheat and its
individual diploid progenitors to know the major di erentially expressed genes (DEGs) involved
during grain development. Two libraries from each species were generated with an average of
55.63, 55.23, 68.13, and 103.81 million reads, resulting in 79.3K, 113.7K, 90.6K, and 121.3K numbers
of transcripts in AA, BB, DD, and AABBDD genome species respectively. Number of expressed
genes in hexaploid wheat was not proportional to its genome size, but marginally higher than that
of its diploid progenitors. However, to capture all the transcripts in hexaploid wheat, su ciently
higher number of reads was required. Functional analysis of DEGs, in all the three comparisons,
showed their predominance in three major classes of genes during grain development, i.e., nutrient
reservoirs, carbohydrate metabolism, and defence proteins; some of them were subsequently validated
through real time quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Further,
developmental stage–specific gene expression showed most of the defence protein genes expressed
during initial developmental stages in hexaploid contrary to the diploids at later stages. Genes related
to carbohydrates anabolism expressed during early stages, whereas catabolism genes expressed at
later stages in all the species. However, no trend was observed in case of di erent nutrient reservoirs
gene expression. This data could be used to study the comparative gene expression among the three
diploid species and homeologue-specific expression in hexaploid.
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Date 2021-07-29T04:27:51Z
2021-07-29T04:27:51Z
2020-05-05
 
Type Article
 
Identifier Kaushik, M., Rai, S., Venkadesan, S., Sinha, S. K., Mohan, S., & Mandal, P. K. (2020). Transcriptome Analysis Reveals Important Candidate Genes Related to Nutrient Reservoir, Carbohydrate Metabolism, and Defence Proteins during Grain Development of Hexaploid Bread Wheat and Its Diploid Progenitors. Genes, 11(5), 509.
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http://krishi.icar.gov.in/jspui/handle/123456789/50903
 
Language English
 
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Publisher Not Available