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A quick, easy and cost-effective in planta method to develop direct transformants in wheat

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Title A quick, easy and cost-effective in planta method to develop direct transformants in wheat
 
Creator Avijit Tarafdar, Harinder Vishwakarma, S Gothandapani, Meenal Bhati, Koushik Biswas, Arul Prakash, Uttara Chaturvedi, Amolkumar U Solanke and Jasdeep Chatrath Padaria
 
Subject Indian bread wheat, Agrobacterium tumefaciens, In planta transformation, Apical meristem, Herbicide tolerance, CP4-EPSPS transgenic wheat
 
Description Not Available
Agrobacterium mediated in planta method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T1 plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated CP4-EPSPS gene, the transgenic populations were screened in T3 generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.
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Date 2021-07-31T06:32:48Z
2021-07-31T06:32:48Z
2019-04-17
 
Type Article
 
Identifier Tarafdar, A., Vishwakarma, H., Gothandapani, S. et al. A quick, easy and cost-effective in planta method to develop direct transformants in wheat. 3 Biotech 9, 180 (2019). https://doi.org/10.1007/s13205-019-1708-6
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http://krishi.icar.gov.in/jspui/handle/123456789/51629
 
Language English
 
Publisher SpringerLink