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Overexpression NPR1 in Brassica juncea confers Broad spectrum resistance to fungal pathogens.

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Title Overexpression NPR1 in Brassica juncea confers Broad spectrum resistance to fungal pathogens.
 
Creator Sajad Ali, Zahoor A. Mir, Anshika Tyagi, Hailay Mehari, Rajendra Meena, Javaid A. Bhat, Prashant Yadav, Pradeep Papalou, Sandhya Rawat, Anita Grover
 
Subject Brassica juncea, NPR1, Alternaria, Powdery mildew, systemic acquired resistance, salicylic acid, jasmonic acid
 
Description Not Available
Brassica juncea (Indian mustard) is a commercially important oil seed crop, which is
highly affected by many biotic stresses. Among them, Alternaria leaf blight and powdery
mildew are the most devastating diseases leading to huge yield losses in B. juncea
around the world. In this regard, genetic engineering is a promising tool that may
possibly allow us to enhance the B. juncea disease resistance against these pathogens.
NPR1 (non-expressor of pathogen-related gene 1) is a bonafide receptor of salicylic
acid (SA) which modulates multiple immune responses in plants especially activation
of induced and systemic acquired resistance (SAR). Here, we report the isolation and
characterization of new NPR1 homolog (BjNPR1) from B. juncea. The phylogenetic
tree constructed based on the deduced sequence of BjNPR1 with homologs from
other species revealed that BjNPR1 grouped together with other known NPR1 proteins
of Cruciferae family, and was nearest to B. napus. Furthermore, expression analysis
showed that BjNPR1 was upregulated after SA treatment and fungal infection but not
by jasmonic acid or abscisic acid. To understand the defensive role of this gene, we
generated B. juncea transgenic lines overexpressing BjNPR1, and further confirmed by
PCR and Southern blotting. The transgenic lines showed no phenotypic abnormalities,
and constitutive expression of BjNPR1 activates defense signaling pathways by priming
the expression of antifungal PR genes. Moreover, BjNPR1 transgenic lines showed
enhanced resistance to Alternaria brassicae and Erysiphe cruciferarum as there was
delay in symptoms and reduced disease severity than non-transgenic plants. In addition,
the rate of disease spreading to uninfected or distal parts was also delayed in transgenic
plants thus suggesting the activation of SAR. Altogether, the present study suggests that
BjNPR1 is involved in broad spectrum of disease resistance against fungal pathogens.
Not Available
 
Date 2021-07-31T06:36:09Z
2021-07-31T06:36:09Z
2017
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/51633
 
Language English
 
Relation Not Available;
 
Publisher Not Available