Sugarcane yellow leaf virus (SCYLV) causing yellow leaf disease in sugarcane: genetic diversity, impact on sugarcane and management
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Title |
Sugarcane yellow leaf virus (SCYLV) causing yellow leaf disease in sugarcane: genetic diversity, impact on sugarcane and management
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Creator |
Viswanathan, R., R. Karuppaiah, P. Malathi, V. Ganesh Kumar and C. Chinnaraja
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Subject |
sugarcane, Sugarcane yellow leaf virus (SCYLV), meristem culture, disease-free planting
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Description |
Not Available
Yellow leaf disease (YLD) caused by Sugarcane yellow leaf virus (SCYLV) is a recently recorded disease in India and is found wide spread throughout country. In popular varieties, the disease incidence varied from 0 to 75.0% and attained epidemic levels under field conditions. Detailed studies on the impact of YLD on sugarcane revealed that the virus infection significantly reduces various cane growth parameters, cane yield and juice quality. Sequence comparisons of the coat protein (CP) and movement protein (MP) of 22 SCYLV isolates from India and database sequences showed a significant variation between Indian isolates and the database sequences both at nt and aa level in the CP/MP coding regions. The significant variation in our isolates with the database isolates, even in the least variable region of the SCYLV genome showed that the population existing in India is different from rest of the world. Further, comparison of partial sequences encoding for ORF 1 and 2 revealed that YLD in sugarcane in India is caused atleast by three genotypes viz., CUB, IND and BRA-PER, of which a majority of the samples were found infected with Cuban genotype (CUB). The genotype IND was identified as a new genotype and this was found to have significant variation with the reported genotypes. We have identified specific primers from CP region of the virus and optimized RT-PCR conditions to diagnose the virus. This assay has been found efficient in detecting the virus in asymptomatic plants and tissue culture derived seedlings. Elimination of the virus through meristem culture has been demonstrated to purify the virus from the infected planting materials and this technique needs to be adopted to supply disease-free planting materials for effective management of the disease. Studies are also in progress to identify the YLD-resistant sources in sugarcane germplasm to initiate breeding for YLD-resistance in sugarcane Not Available |
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Date |
2021-11-27T08:34:00Z
2021-11-27T08:34:00Z 2010-09-18 |
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Type |
Presentation
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Identifier |
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2347 3584 19 th National Conference on "Recent Trends in Viral Disease Problems and Management" http://krishi.icar.gov.in/jspui/handle/123456789/67862 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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