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Separation and quantification of vitamin E isoforms through ion chromatography in groundnut oil

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Title Separation and quantification of vitamin E isoforms through ion chromatography in groundnut oil
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Creator Aman Verma
M.K. Mahatma
L.K. Thawait
Sushmita Singh
Rajaram Choudhary
Devendra Singh
 
Subject Tocopherols
groundnut oil
Ion chromatography
 
Description Not Available
A convenient analytic method was developed for measuring major vitamins E isomeric forms (α-, β-, γ- and δ-Tocopherol) in groundnut oil. A 5 µm Polar Advantage II, 120 Å, C-18 column, with the dimensions of 4.6 × 150 mm and maintained at 25 °C, is used to resolve vitamin E. The separation starts with a 5.5 min composition hold with absolute methanol as mobile phase with flow rate of 1.0 ml/min, followed by reduction in flow rate i.e. 0.4 ml/min for up to 9.0 min and 0.6 ml/min for last one minute. Detection is accomplished using UV detector at wavelength 295 nm. The elution order of tocopherols in TG-51 genotype was δ-tocopherol, (β+γ)- tocopherol and α-tocopherol with retention time of 4.87, 6.01 and 6.43 minutes respectively. The total tocopherol content in TG-51 was found to be 567 ppm with 49 ppm of δ-tocopherol, 109 ppm of β+γ-tocopherol and 409 ppm of α-tocopherol. Peaks of α-, β-, γ- and δ-Tocopherol in groundnut oil were identified by comparing their retention times with commercially available standards (Sigma–Aldrich, St. Louis, MO, USA).
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Date 2022-01-21T11:15:06Z
2022-01-21T11:15:06Z
2021-12-13
 
Type Other
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/68869
 
Language English
 
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Publisher Not Available