ICAR Research Data Repository for Knowledge Management
Computer program for DNA marker discovery
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Computer program for DNA marker discovery
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| Creator |
Arunachalam, V.,
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| Subject |
ISCAR marker; DNA; Priming sites; RAPD; ISSR;
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| Description |
Not Available
Conventional Random Amplification of Polymorphic DNA and Inter Simple Sequence Repeat molecular markers work on the random process, and their success depends on the availability of the priming sites on the sequences. Failure due to inappropriate primers can cause a researcher frustrated due to waste of chemicals and time. Failure due to inappropriate primers can cost as high US$ 100 (INR 7200) for a small experiment of 20 reactions. Thousands of such experiments are conducted using polymerase chain reactions (PCR) machines worldwide every year. The software works by following simple steps: searches for priming sites of the given primer(s) in all the target sequences, calculates priming sites of sequence(s), measures distance between priming sites, and converts best amplified primers into iSCAR primers. Not Available |
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| Date |
2022-01-18T10:19:11Z
2022-01-18T10:19:11Z 2020-06-06 |
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| Type |
Success Story
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/68787 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Not Available
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