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The effects of methanolic extract of <em>Uncaria gambir</em> against microflora of dental caries

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Title Statement The effects of methanolic extract of <em>Uncaria gambir</em> against microflora of dental caries
 
Added Entry - Uncontrolled Name Ahmad Murad, Nur Fazwani ; Department of Paediatric Dentistry, Selayang Hospital, Lebuhraya Selayang-Kepong, 68100 Batu Caves, Selangor, Malaysia
Mahyuddin, Alida ; Department of Family Oral Health, Faculty of Dentistry, The National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Shafiei, Zaleha ; Department of Craniofacial Diagnostic and Biosciences, Faculty of Dentistry, The National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Sockalingam, S Nagarajan MP; Department of Family Oral Health, Faculty of Dentistry, The National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Zakaria, Ahmad Shuhud Irfani; Department of Family Oral Health, Faculty of Dentistry, The National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
 
Uncontrolled Index Term microbiology; dental caries; natural products
Antibacterial; Enterococcus faecalis; Lactobacillus casei; Streptococcus mutans; Streptococcus sobrinus; Uncaria gambir
 
Summary, etc. <p style="text-align: justify;">The antibacterial effects of <em>Uncaria gambir</em> extract have the potential to be expanded in dentistry, mainly in the management of dental caries and its sequelae. This preliminary study was conducted to investigate the <em>in vitro</em> antibacterial activity of methanolic extract of <em>U. gambir</em> against dental caries-related microflora: <em>Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei</em> and <em>Enterococcus faecalis.</em> The methanolic extract of <em>U. gambir</em> in powder form was dissolved and serially diluted in 1% dimethyl sulfoxide. The antibacterial effects of the extract were determined using the broth microdilution technique. A transmission electron microscope (TEM) was used to assess the effects of the extract on the morphology of the bacteria. A 0.12% chlorhexidine (CHX) and Man-Rogosa-Sharpe/brain heart infusion broth were used as positive and negative control respectively. Greatest antibacterial effects were seen on both Streptococci species with the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of 1.25 and 5 mg/mL respectively, followed by <em>Enterococcus faecalis</em> (MIC=2.5 mg/mL, MBC=10 mg/mL) and <em>Lactobacillus casei</em> (MIC=7.5 mg/mL, MBC=30 mg/mL) in ascending order. Cell wall damage of all bacteria at their respective MIC value was observed through the TEM analysis. Tukey’s posthoc test showed no statistically significant difference in the antibacterial activity exerted by <em>U. gambir</em> extract and 0.12% CHX, with <em>P</em>&gt; 0.05. Conclusively, <em>U. gambir </em>extract exhibits a good antibacterial effect against the microflora of dental caries and carries great potential for future development.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2022-12-05 09:59:16
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/62698
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 13, ##issue.no## 4 (2022): December 2022 (Ahead of Print)
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJNPR/article/download/62698/465606737
http://op.niscair.res.in/index.php/IJNPR/article/download/62698/465606739
 
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